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Determining Antiradical Capacity of Medicinal Plant Extract Individual Constituents Using Post-Column Reaction Method.
Przybyl, Jaroslaw L; Stefaniak, Jan; Jaroszewicz, Anna; Gawronska, Amanda; Lapinski, Marcin; Baczek, Katarzyna Barbara; Weglarz, Zenon.
Afiliación
  • Przybyl JL; Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warszawa, Poland.
  • Stefaniak J; Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warszawa, Poland.
  • Jaroszewicz A; Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warszawa, Poland.
  • Gawronska A; Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warszawa, Poland.
  • Lapinski M; Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warszawa, Poland.
  • Baczek KB; Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warszawa, Poland.
  • Weglarz Z; Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warszawa, Poland.
Int J Mol Sci ; 25(10)2024 May 17.
Article en En | MEDLINE | ID: mdl-38791498
ABSTRACT
The post-column reaction method enables the evaluation of the antiradical capacity of individual components in a mixture by separating the components using HPLC and measuring stable free radical (e.g., DPPH●) scavenging that occurs after the chromatography column. The equipment typically consists of two detectors. The first records signals of the analytes leaving the column. The second records radical scavenging by the analytes, which appears as a negative band. The recorded signals are found on two separate chromatograms, which must be combined to interpret the results. In this study, a single DAD detector was used behind the post-column reactor, enabling the simultaneous recording of the analyte bands and negative signals, indicating radical scavenging. The objective of this study was to evaluate the antiradical capacity of key compounds found in two herbal raw materials used in traditional Chinese medicine. Saposhnikovia divaricata roots contain phenolic acids, chromones, and furanocoumarins. Chlorogenic acid, rosmarinic acid, and imperatorin demonstrated strong radical scavenging, while prim-O-glucoslocimifugin showed a weaker response, both in standards and in root extracts. However, scavenging was not observed for cimifugin and 4'-O-ß-D-glucosyl-5-O-methylvisamminol. Astragalus mongholicus roots contain astragalosides I-IV (triterpene saponins). None of these showed DPPH● scavenging. Furthermore, additional signals were observed, indicating the presence of unidentified radical scavenging compounds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas Medicinales / Extractos Vegetales / Depuradores de Radicales Libres Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas Medicinales / Extractos Vegetales / Depuradores de Radicales Libres Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza