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Comparative Mitogenome of Phylogenetic Relationships and Divergence Time Analysis within Potamanthidae (Insecta: Ephemeroptera).
Guo, Zhi-Qiang; Gao, Ya-Jie; Chen, Yu-Xin; Zhan, Le-Mei; Storey, Kenneth B; Yu, Dan-Na; Zhang, Jia-Yong.
Afiliación
  • Guo ZQ; College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
  • Gao YJ; School of Bioengineering, Aksu Vocational Technical College, Aksu 843000, China.
  • Chen YX; College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
  • Zhan LM; College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
  • Storey KB; Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada.
  • Yu DN; College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
  • Zhang JY; Key Lab of Wildlife Biotechnology, Covnservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, China.
Insects ; 15(5)2024 May 15.
Article en En | MEDLINE | ID: mdl-38786913
ABSTRACT
Potamanthidae belongs to the superfamily Ephemeroidea but has no complete mt genome released in the NCBI (except for two unchecked and one partial mt genome). Since the sister clade to Potamanthidae has always been controversial, we sequenced seven mt genomes of Potamanthidae (two species from Rhoenanthus and five species from Potamanthus) in order to rebuild the phylogenetic relationships of Potamanthidae in this study. The divergence time of Potamanthidae was also investigated by utilizing five fossil calibration points because of the indeterminate origin time. In addition, because Rhoenanthus coreanus and Potamanthus luteus are always in low-temperature environments, we aimed to explore whether these two species were under positive selection at the mt genome level. Amongst the 13 PCGs, CGA was used as the start codon in COX1, whereas other genes conformed to initiating with an ATN start codon. From this analysis, UUA (L), AUU (I), and UUU (F) had the highest usage. Furthermore, the DHU arm was absent in the secondary structure of S1 in all species. By combining the 13 PCGs and 2 rRNAs, we reconstructed the phylogenetic relationship of Potamanthidae within Ephemeroptera. The monophyly of Potamanthidae and the monophyly of Rhoenanthus and Potamanthus were supported in the results. The phylogenetic relationship of Potamanthidae + (Ephemeridae + Polymitarcyidae) was also recovered with a high prior probability. The divergence times of Potamanthidae were traced to be 90.44 Mya (95% HPD, 62.80-121.74 Mya), and the divergence times of Rhoenanthus and Potamanthus originated at approximately 64.77 Mya (95% HPD, 43.82-88.68 Mya), thus belonging to the late Pliocene Epoch or early Miocene Epoch. In addition, the data indicated that R. coreanus was under negative selection and that ATP8 and ND2 in Potamanthidae had a high evolutionary rate.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Insects Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Insects Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza