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Detection of Glutamate Decarboxylase Antibodies and Simultaneous Multi-Molecular Translocation Exploration by Glass Nanopores.
Tao, Chongxin; Bai, Yun; Chen, Jiang; Lu, Jing; Bi, Yan; Li, Jian.
Afiliación
  • Tao C; Key Laboratory of DGHD, MOE, School of Life Science and Technology, Southeast University, Nanjing 210096, China.
  • Bai Y; Key Laboratory of DGHD, MOE, School of Life Science and Technology, Southeast University, Nanjing 210096, China.
  • Chen J; Key Laboratory of DGHD, MOE, School of Life Science and Technology, Southeast University, Nanjing 210096, China.
  • Lu J; Department of Endocrinology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China.
  • Bi Y; Department of Endocrinology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China.
  • Li J; Key Laboratory of DGHD, MOE, School of Life Science and Technology, Southeast University, Nanjing 210096, China.
Biosensors (Basel) ; 14(5)2024 May 17.
Article en En | MEDLINE | ID: mdl-38785729
ABSTRACT
Glutamic acid decarboxylase antibody (GADAb) has emerged as a significant biomarker for clinical diagnosis and prognosis in type 1 diabetes (T1D). In this study, we investigated the potential utilization of glass capillary solid-state nanopores as a cost-effective and easily preparable platform for the detection of individual antigens, antibodies, and antigen-antibody complexes without necessitating any modifications to the nanopores. Our findings revealed notable characteristic variations in the translocation events of glutamic acid decarboxylase (GAD65) through nanopores under different voltage conditions, discovered that anomalous phenomenon of protein translocation events increasing with voltage may potentially be caused by the crowding of multiple proteins in the nanopores, and demonstrated that there are multiple components in the polyclonal antibodies (GADAb-poly). Furthermore, we achieved successful differentiation between GAD65, GADAb, and GADAb-GAD65 complexes. These results offer promising prospects for the development of a rapid and reliable GADAb detection method, which holds the potential to be applied in patient serum samples, thereby facilitating a label-free, cost-effective, and early diagnosis of type I diabetes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diabetes Mellitus Tipo 1 / Nanoporos / Glutamato Descarboxilasa Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diabetes Mellitus Tipo 1 / Nanoporos / Glutamato Descarboxilasa Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza