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An Innovative Approach for Tailoring Molecularly Imprinted Polymers for Biosensors-Application to Cancer Antigen 15-3.
Dos Santos Oliveira, Daniela; Oliveira, Andreia Sofia Rodrigues; Mendonça, Patrícia Vitorino; Coelho, Jorge Fernando Jordão; Moreira, Felismina Teixeira Coelho; Sales, Maria Goreti Ferreira.
Afiliación
  • Dos Santos Oliveira D; BioMark@ISEP-CEB/LABBELS, School of Engineering, Polytechnic Institute of Porto, R. Dr. António Bernardino de Almeida, 4249-015 Porto, Portugal.
  • Oliveira ASR; Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Pole II, Rua Sílvio Lima, 3030-790 Coimbra, Portugal.
  • Mendonça PV; Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Pole II, Rua Sílvio Lima, 3030-790 Coimbra, Portugal.
  • Coelho JFJ; Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Pole II, Rua Sílvio Lima, 3030-790 Coimbra, Portugal.
  • Moreira FTC; Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Pole II, Rua Sílvio Lima, 3030-790 Coimbra, Portugal.
  • Sales MGF; Instituto Pedro Nunes (IPN), Associação para a Inovação e Desenvolvimento em Ciência e Tecnologia, R. Pedro Nunes, 3030-199 Coimbra, Portugal.
Biosensors (Basel) ; 14(5)2024 Apr 30.
Article en En | MEDLINE | ID: mdl-38785696
ABSTRACT
This work presents a novel approach for tailoring molecularly imprinted polymers (MIPs) with a preliminary stage of atom transfer radical polymerization (ATRP), for a more precise definition of the imprinted cavity. A well-defined copolymer of acrylamide and N,N'-methylenebisacrylamide (PAAm-co-PMBAm) was synthesized by ATRP and applied to gold electrodes with the template, followed by a crosslinking reaction. The template was removed from the polymer matrix by enzymatic/chemical action. The surface modifications were monitored via electrochemical impedance spectroscopy (EIS), having the MIP polymer as a non-conducting film designed with affinity sites for CA15-3. The resulting biosensor exhibited a linear response to CA15-3 log concentrations from 0.001 to 100 U/mL in PBS or in diluted fetal bovine serum (1000×) in PBS. Compared to the polyacrylamide (PAAm) MIP from conventional free-radical polymerization, the ATRP-based MIP extended the biosensor's dynamic linear range 10-fold, improving low concentration detection, and enhanced the signal reproducibility across units. The biosensor demonstrated good sensitivity and selectivity. Overall, the work described confirmed that the process of radical polymerization to build an MIP material influences the detection capacity for the target substance and the reproducibility among different biosensor units. Extending this approach to other cancer biomarkers, the methodology presented could open doors to a new generation of MIP-based biosensors for point-of-care disease diagnosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Polimerizacion / Polímeros Impresos Molecularmente Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Polimerizacion / Polímeros Impresos Molecularmente Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Suiza