Effects of dietary fiber and biotic supplementation on apparent total tract macronutrient digestibility and the fecal characteristics, metabolites, and microbiota of healthy adult dogs.
J Anim Sci
; 1022024 Jan 03.
Article
en En
| MEDLINE
| ID: mdl-38783711
ABSTRACT
Dietary fibers and biotics have been shown to support gastrointestinal health in dogs, but are usually tested individually. There is value in testing fiber-biotic combinations that are commonly used commercially. Therefore, this study was conducted to determine the apparent total tract macronutrient digestibility (ATTD) of diets supplemented with fibers or biotics and to evaluate their effects on the fecal characteristics, metabolites, microbiota, and immunoglobulin A (IgA) concentrations of dogs. Twelve healthy adult female beagle dogs (ageâ
=â
6.2â
±â
1.6 yr; body weightâ
=â
9.5â
±â
1.1 kg) were used in a replicated 3â
×â
3 Latin square design to test three treatments 1) control diet based on rice, chicken meal, tapioca starch, and celluloseâ
+â
a placebo treat (CT); 2) diet based on rice, chicken meal, garbanzo beans, and celluloseâ
+â
a placebo treat (GB); 3) diet based on rice, chicken meal, garbanzo beans, and a functional fiber/prebiotic blendâ
+â
a probiotic-containing treat (GBPP). In each 28-d period, a 22-d diet adaptation was followed by a 5-d fecal collection phase. Fasted blood samples were collected on day 28. Data were analyzed using the Mixed Models procedure of SAS 9.4, with Pâ
<â
0.05 being significant and Pâ
<â
0.10 being trends. ATTD of dry matter (DM), organic matter, and energy were lower (Pâ
<â
0.001) and DM fecal output was higher (Pâ
<â
0.01) in dogs fed GBPP than CT or GB, whereas ATTD of crude protein was higher (Pâ
<â
0.001) in dogs fed CT and GBPP than GB. ATTD of fat was higher (Pâ
<â
0.001) and wet fecal output was lower (Pâ
<â
0.01) in dogs fed CT than GB or GBPP. Fecal DM% was higher (Pâ
<â
0.001) in dogs fed CT than GBPP or GB, and higher in dogs fed GBPP than GB. Fecal short-chain fatty acid concentrations were higher (Pâ
<â
0.001) in dogs fed GB than CT or GBPP, and higher in dogs fed GB than GBPP. Fecal IgA concentrations were higher (Pâ
<â
0.01) in dogs fed GB than CT. Fecal microbiota populations were affected by diet, with alpha diversity being higher (Pâ
<â
0.01) in dogs fed GB than CT, and beta diversity shifting following dietary fiber and biotic supplementation. The relative abundance of 24 bacterial genera was altered in dogs fed GB or GBPP than CT. Serum triglyceride concentrations were lower in dogs fed GB than GBPP or CT. Our results demonstrate that legume-based dietary fibers, with or without prebiotics and probiotics, reduce ATTD, increase stool output, beneficially shift fecal metabolites and microbiota, and reduce blood lipids in adult dogs.
Functional fibers and biotics have demonstrated the potential to modulate the gut microbiome and improve gastrointestinal health in dogs, but are often tested individually. Research investigating unique fiber/biotic combinations is needed. Twelve dogs were used in a replicated 3â
×â
3 Latin square design to test the efficacy of three dietary treatments on apparent total tract macronutrient digestibility (ATTD) and the fecal characteristics, metabolites, microbiota, and immunoglobulin A concentrations of dogs. Treatments included a low-fiber control diet without prebiotics or probioticsâ
+â
a placebo treat, a diet containing garbanzo beansâ
+â
a placebo treat (GB), and a diet containing garbanzo beans and a prebiotic blendâ
+â
a probiotic (Bacillus subtilis and Bacillus amyloliquefaciens) treat (GBPP). ATTD was reduced and stool output was greater in dogs fed GB or GBPP than controls. Fecal short-chain fatty acids were higher in dogs fed GB or GBPP than controls. Fecal immunoglobulin A was higher, while blood lipids were lower in dogs fed GB than control. Finally, GB and GBPP shifted fecal bacterial populations. Our results demonstrate that legume-based dietary fibers, with or without prebiotics and probiotics, reduce ATTD, increase stool output, beneficially shift fecal metabolites and microbiota, and reduce blood lipids in adult dogs.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fibras de la Dieta
/
Suplementos Dietéticos
/
Dieta
/
Digestión
/
Heces
/
Microbioma Gastrointestinal
/
Alimentación Animal
Límite:
Animals
Idioma:
En
Revista:
J Anim Sci
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos