The basic functions of rabbit ovarian granulosa cell are regulated by adipokines monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1.
Domest Anim Endocrinol
; 88: 106856, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38781776
ABSTRACT
The aim of the present study was to examine the influence of monocyte chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1) on ovarian cell functions. Rabbit ovarian granulosa cells were cultured with or without MCP-1 or PAI-1 (at 0, 0.1, 1, or 10 ng/ml). Cell viability, proliferation, cytoplasmic apoptosis and release of progesterone and estradiol were measured by Cell Counting Kit-8 (CCK-8), BrdU incorporation, and cell death detection assays and ELISA. The addition of either MCP-1 or PAI-1 increased cell viability and proliferation and decreased apoptosis. MCP-1 promoted, while PAI-1 suppressed, progesterone release. Both MCP-1 and PAI-1 reduced estradiol output. The present results suggest that MCP-1 or PAI-1 can be physiological promoters of rabbit ovarian cell viability and proliferation, inhibitors of apoptosis and regulators of ovarian steroidogenesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Progesterona
/
Inhibidor 1 de Activador Plasminogénico
/
Apoptosis
/
Quimiocina CCL2
/
Células de la Granulosa
Límite:
Animals
Idioma:
En
Revista:
Domest Anim Endocrinol
Asunto de la revista:
ENDOCRINOLOGIA
/
MEDICINA VETERINARIA
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Estados Unidos