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Does PAD and microcirculation status impact the tissue availability of intravenously administered antibiotics in patients with infected diabetic foot? Results of the DFIATIM substudy.
Fejfarová, Vladimíra; Jarosíková, Radka; Antalová, Simona; Husáková, Jitka; Wosková, Veronika; Beca, Pavol; Mrázek, Jakub; Tuma, Petr; Polák, Jan; Dubský, Michal; Sojáková, Dominika; Lánská, Vera; Petrlík, Martin.
Afiliación
  • Fejfarová V; Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czechia.
  • Jarosíková R; Department of Internal Medicine, Second Faculty of Medicine, Charles University, Prague, Czechia.
  • Antalová S; Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czechia.
  • Husáková J; Department of Internal Medicine, Second Faculty of Medicine, Charles University, Prague, Czechia.
  • Wosková V; Department of Clinical Pharmacy and Drug Information Centre, Institute for Clinical and Experimental Medicine, Prague, Czechia.
  • Beca P; Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czechia.
  • Mrázek J; Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czechia.
  • Tuma P; Department of Clinical Pharmacy and Drug Information Centre, Institute for Clinical and Experimental Medicine, Prague, Czechia.
  • Polák J; Laboratory of Anaerobic Microbiology, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Prague, Czechia.
  • Dubský M; Department of Hygiene, Third Faculty of Medicine, Charles University, Prague, Czechia.
  • Sojáková D; Department of Pathophysiology, Third Faculty of Medicine, Charles University, Prague, Czechia.
  • Lánská V; Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czechia.
  • Petrlík M; Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czechia.
Front Endocrinol (Lausanne) ; 15: 1326179, 2024.
Article en En | MEDLINE | ID: mdl-38774229
ABSTRACT
Aims/

hypothesis:

The aim of this substudy (Eudra CT No2019-001997-27)was to assess ATB availability in patients with infected diabetic foot ulcers(IDFUs)in the context of microcirculation and macrocirculation status.

Methods:

For this substudy, we enrolled 23 patients with IDFU. Patients were treated with boluses of amoxicillin/clavulanic acid(AMC)(12patients) or ceftazidime(CTZ)(11patients). After induction of a steady ATB state, microdialysis was performed near the IDFU. Tissue fluid samples from the foot and blood samples from peripheral blood were taken within 6 hours. ATB potential efficacy was assessed by evaluating the maximum serum and tissue ATB concentrations(Cmax and Cmax-tissue)and the percentage of time the unbound drug tissue concentration exceeds the minimum inhibitory concentration (MIC)(≥100% tissue and ≥50%/60% tissue fT>MIC). Vascular status was assessed by triplex ultrasound, ankle-brachial and toe-brachial index tests, occlusive plethysmography comprising two arterial flow phases, and transcutaneous oxygen pressure(TcPO2).

Results:

Following bolus administration, the Cmax of AMC was 91.8 ± 52.5 µgmL-1 and the Cmax-tissue of AMC was 7.25 ± 4.5 µgmL-1(P<0.001). The Cmax for CTZ was 186.8 ± 44.1 µgmL-1 and the Cmax-tissue of CTZ was 18.6 ± 7.4 µgmL-1(P<0.0001). Additionally, 67% of patients treated with AMC and 55% of those treated with CTZ achieved tissue fT>MIC levels exceeding 50% and 60%, respectively. We observed positive correlations between both Cmax-tissue and AUCtissue and arterial flow. Specifically, the correlation coefficient for the first phase was r=0.42; (P=0.045), and for the second phase, it was r=0.55(P=0.01)and r=0.5(P=0.021).

Conclusions:

Bactericidal activity proved satisfactory in only half to two-thirds of patients with IDFUs, an outcome that appears to correlate primarily with arterial flow.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pie Diabético / Microcirculación / Antibacterianos Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Front Endocrinol (Lausanne) Año: 2024 Tipo del documento: Article Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pie Diabético / Microcirculación / Antibacterianos Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Front Endocrinol (Lausanne) Año: 2024 Tipo del documento: Article Pais de publicación: Suiza