Your browser doesn't support javascript.
loading
Protocol for separation of fungal extracellular vesicles using ultracentrifugation from solid medium cultures.
Piffer, Alicia C; Reis, Flavia C G; Moyrand, Frédérique; Montalvão, Bruna; Rodrigues, Marcio L; Janbon, Guilhem; Rizzo, Juliana.
Afiliación
  • Piffer AC; Institut Pasteur, Université Paris Cité, Unité Biologie des ARN des Pathogènes Fongiques, Département de Mycologie, 75015 Paris, France. Electronic address: alicia.corbellini-piffer@pasteur.fr.
  • Reis FCG; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba 81350-010, Brazil; Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Fiocruz, Rio de Janeiro 21040-361, Brazil.
  • Moyrand F; Institut Pasteur, Université Paris Cité, Unité Biologie des ARN des Pathogènes Fongiques, Département de Mycologie, 75015 Paris, France.
  • Montalvão B; Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil; Instituto de Biofísica Carlos Chagas Filho (IBCCF), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-904, Brazil.
  • Rodrigues ML; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba 81350-010, Brazil; Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil.
  • Janbon G; Institut Pasteur, Université Paris Cité, Unité Biologie des ARN des Pathogènes Fongiques, Département de Mycologie, 75015 Paris, France.
  • Rizzo J; Instituto de Biofísica Carlos Chagas Filho (IBCCF), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-904, Brazil. Electronic address: juliana.rizzo@biof.ufrj.br.
STAR Protoc ; 5(2): 103069, 2024 Jun 21.
Article en En | MEDLINE | ID: mdl-38771694
ABSTRACT
Extracellular vesicles (EVs) have been identified in diverse fungi, including human pathogens. In this protocol, we present two techniques for isolating and analyzing fungal EVs. The first is for high-throughput screening, and the second is for yielding concentrated samples suitable for centrifugation-based density gradients. We describe steps for analytical assays such as nano-flow cytometry and nanoparticle tracking analysis to measure EV dimensions and concentration. EV suspensions can serve diverse assays, including electron microscopy, compositional determination, and cell-to-cell communication assays. For complete details on the use and execution of this protocol, please refer to Rizzo et al.,1 Rizzo et al.,2 Reis et al.,3 and Reis et al.4.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ultracentrifugación / Vesículas Extracelulares / Hongos Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ultracentrifugación / Vesículas Extracelulares / Hongos Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos