Your browser doesn't support javascript.
loading
Anthelminthic and antimicrobial effects of hedge woundwort (Stachys sylvatica L.) growing in Southern Kazakhstan.
Mukhamedsadykova, Aigerim Z; Kasela, Martyna; Kozhanova, Kaldanay K; Sakipova, Zuriyadda B; Kukula-Koch, Wirginia; Józefczyk, Aleksandra; Swiatek, Lukasz; Rajtar, Barbara; Iwan, Magdalena; Kolodziej, Przemyslaw; Ludwiczuk, Agnieszka; Kadyrbayeva, Gulnara M; Kuntubek, Gulnur N; Mamatova, Aliya S; Bogucka-Kocka, Anna; Malm, Anna.
Afiliación
  • Mukhamedsadykova AZ; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, Almaty, Kazakhstan.
  • Kasela M; Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.
  • Kozhanova KK; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, Almaty, Kazakhstan.
  • Sakipova ZB; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, Almaty, Kazakhstan.
  • Kukula-Koch W; Department of Pharmacognosy, Medical University of Lublin, Lublin, Poland.
  • Józefczyk A; Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, Lublin, Poland.
  • Swiatek L; Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, Lublin, Poland.
  • Rajtar B; Department of Virology with Viral Diagnostics Laboratory, Medical University of Lublin, Lublin, Poland.
  • Iwan M; Department of Toxicology, Medical University of Lublin, Lublin, Poland.
  • Kolodziej P; Department of Biology and Genetics, Medical University of Lublin, Lublin, Poland.
  • Ludwiczuk A; Department of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, Lublin, Poland.
  • Kadyrbayeva GM; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, Almaty, Kazakhstan.
  • Kuntubek GN; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, Almaty, Kazakhstan.
  • Mamatova AS; Department of Microbiology and Virology, Kazakh National Medical University, Almaty, Kazakhstan.
  • Bogucka-Kocka A; Department of Biology and Genetics, Medical University of Lublin, Lublin, Poland.
  • Malm A; Department of Pharmaceutical Microbiology, Medical University of Lublin, Lublin, Poland.
Front Pharmacol ; 15: 1386509, 2024.
Article en En | MEDLINE | ID: mdl-38769997
ABSTRACT
The Stachys L. genus has been widely used in traditional medicine in many countries throughout the world. The study aimed to investigate the chemical composition and bioactivity of the hydroethanolic extract (50% v/v) obtained by ultrasonication from the aerial flowering parts of Stachys sylvatica L. (SSE) collected in Almaty region (Southern Kazakhstan). According to RP-HPLC/PDA analysis the leading metabolites of the SSE belonged to polyphenols chlorogenic acid and its isomers (2.34 mg/g dry extract) and luteolin derivatives (1.49 mg/g dry extract), while HPLC-ESI-QTOF-MS/MS-based qualitative fingerprinting revealed the presence of 17 metabolites, mainly chlorogenic acid and its isomers, flavonoid glycosides, and verbascoside with its derivatives. GC-MS analysis of the volatile metabolites showed mainly the presence of diterpenoids and fatty acid esters. A reduction in the viability of nematodes Rhabditis sp. was obtained for the SSE concentration of 3.3 mg/mL, while 11.1 mg/mL showed activity comparable to albendazole. The SSE exhibited higher activity against Gram-positive (MIC = 0.5-2 mg/mL) than Gram-negative bacteria and yeast (MIC = 8 mg/mL), exerting bactericidal and fungicidal effects but with no sporicidal activity. The SSE showed some antiviral activity against HCoV-229E replicating in MRC-5 and good protection against the cytopathic effect induced by HHV-1 in VERO. The SSE was moderately cytotoxic towards human cervical adenocarcinoma (H1HeLa) cells (CC50 of 0.127 mg/mL after 72 h). This study provides novel information on the SSE extract composition and its biological activity, especially in the context of the SSE as a promising candidate for further antiparasitic studies.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: Kazajstán Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: Kazajstán Pais de publicación: Suiza