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A validated ultra-performance liquid chromatography with tandem mass spectrometry method for the quantification of Brazilian green propolis main compounds.
Santos, Mário Ferreira Conceição; Mirada, Giulia Stavrakas; do Couto, Joice Olinda; de Oliveira Costa, Gilvana; Rangel Rosa, Ana Carla; Gambeta Borges, Carly Henrique; Crevelin, Eduardo José; de Araújo, Luciana Silva; Bastos, Jairo K; Veneziani, Rodrigo C S; Ambrósio, Sérgio R.
Afiliación
  • Santos MFC; Center of Exact, Natural and Health Sciences, Federal University of Espírito Santo - UFES, Alto Universitário, Alegre, Espírito Santo, Brazil.
  • Mirada GS; Center of Exact, Natural and Health Sciences, Federal University of Espírito Santo - UFES, Alto Universitário, Alegre, Espírito Santo, Brazil.
  • do Couto JO; Center for Agricultural Engineering Sciences, Federal University of Espírito Santo - UFES, Alto Universitário, Alegre, Espírito Santo, Brazil.
  • de Oliveira Costa G; Center of Exact, Natural and Health Sciences, Federal University of Espírito Santo - UFES, Alto Universitário, Alegre, Espírito Santo, Brazil.
  • Rangel Rosa AC; Center of Exact, Natural and Health Sciences, Federal University of Espírito Santo - UFES, Alto Universitário, Alegre, Espírito Santo, Brazil.
  • Gambeta Borges CH; Research Center for Exact and Technological Sciences, University of Franca, Franca, SP, Brazil.
  • Crevelin EJ; Department of Chemistry, Faculty of Philosophy, Science and Letters of Ribeirão Preto Faculty of Philosophy, Science and Letters of Ribeirão Preto, University of São Paulo,University of São Paulo, Ribeirão Preto, Brazil.
  • de Araújo LS; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
  • Bastos JK; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil.
  • Veneziani RCS; Research Center for Exact and Technological Sciences, University of Franca, Franca, SP, Brazil.
  • Ambrósio SR; Research Center for Exact and Technological Sciences, University of Franca, Franca, SP, Brazil.
Nat Prod Res ; : 1-7, 2024 May 20.
Article en En | MEDLINE | ID: mdl-38768436
ABSTRACT
Brazilian green propolis is used in folk medicine because of its various biological properties. The hydroalcoholic extract of Brazilian green propolis is characteristic for possessing several pharmacological properties. Phytochemical investigations have attributed some of these properties to the presence of compounds, which were chosen as analytical markers. This paper reports the development and analytical validation using UPLC-MS/MS in MRM mode. Veratraldehyde was used as an internal standard in qualitative and quantitative analyses of the extracts. Relative standard deviation values obtained for intra-day and inter-day precision were lower than 4%. Of the five parameters for robustness, wavelength detection and flow rate were the critical ones. Limits of detection and quantification ranged from 0.300 to 39.500 ng.mL-1 and from 1.400 to 85.00 ng.mL-1, respectively. The recoveries were between 94.00 and 119.00%, with relative standard deviation values around 5.0%. The developed method is precise, sensitive, and reliable for analysing Brazilian green propolis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE País/Región como asunto: America do sul / Brasil Idioma: En Revista: Nat Prod Res Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE País/Región como asunto: America do sul / Brasil Idioma: En Revista: Nat Prod Res Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido