Eukaryotic Ribosome Assembly.

Vanden Broeck, Arnaud; Klinge, Sebastian

Vanden Broeck, Arnaud; Klinge, Sebastian.

Afiliación

Vanden Broeck A; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, New York, USA; email: klinge@rockefeller.edu.
Klinge S; Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, New York, USA; email: klinge@rockefeller.edu.

Annu Rev Biochem ; 93(1): 189-210, 2024 Aug.

Article en En | MEDLINE | ID: mdl-38768392

ABSTRACT

ABSTRACT

During the last ten years, developments in cryo-electron microscopy have transformed our understanding of eukaryotic ribosome assembly. As a result, the field has advanced from a list of the vast array of ribosome assembly factors toward an emerging molecular movie in which individual frames are represented by structures of stable ribosome assembly intermediates with complementary biochemical and genetic data. In this review, we discuss the mechanisms driving the assembly of yeast and human small and large ribosomal subunits. A particular emphasis is placed on the most recent findings that illustrate key concepts of ribosome assembly, such as folding of preribosomal RNA, the enforced chronology of assembly, enzyme-mediated irreversible transitions, and proofreading of preribosomal particles.

Asunto(s)

Microscopía por Crioelectrón; Proteínas Ribosómicas; Ribosomas; Humanos; Ribosomas/metabolismo; Ribosomas/ultraestructura; Ribosomas/química; Ribosomas/genética; Proteínas Ribosómicas/metabolismo; Proteínas Ribosómicas/química; Proteínas Ribosómicas/genética; ARN Ribosómico/metabolismo; ARN Ribosómico/química; ARN Ribosómico/genética; Saccharomyces cerevisiae/metabolismo; Saccharomyces cerevisiae/genética; Modelos Moleculares; Células Eucariotas/metabolismo; Células Eucariotas/ultraestructura; Pliegue del ARN; Subunidades Ribosómicas Pequeñas de Eucariotas/metabolismo; Subunidades Ribosómicas Pequeñas de Eucariotas/química; Subunidades Ribosómicas Pequeñas de Eucariotas/genética; Subunidades Ribosómicas Pequeñas de Eucariotas/ultraestructura; Animales

Palabras clave

RNA folding; cryoelectron microscopy; eukaryotic ribosome; rRNA processing; ribosome assembly; ribosome biogenesis

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Ribosómicas / Ribosomas / Microscopía por Crioelectrón Límite: Animals / Humans Idioma: En Revista: Annu Rev Biochem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos

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