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DNA Extraction and Comparison between Old and Fresh Necrophilic Fly Samples.
Wang, Bo; Xu, Yu-Zhao; Zhou, Yun-Chao; Ha, Shan; Qin, Xiao-Shi; Cai, Jie; Cong, Bin; Chen, Jian-Hua; Deng, Jian-Qiang.
Afiliación
  • Wang B; Hainan Vocational College of Political Science and Law, Hainan Gongping Forensic Center.
  • Xu YZ; Hainan Province Tropical Forensic Engineering Research Center, Department of Forensic Medicine, Hainan Medical University, Hainan Provincial Academician Workstation (Tropical Forensic Medicine).
  • Zhou YC; Hainan Province Tropical Forensic Engineering Research Center, Department of Forensic Medicine, Hainan Medical University, Hainan Provincial Academician Workstation (Tropical Forensic Medicine).
  • Ha S; Hainan Province Tropical Forensic Engineering Research Center, Department of Forensic Medicine, Hainan Medical University, Hainan Provincial Academician Workstation (Tropical Forensic Medicine).
  • Qin XS; Hainan Province Tropical Forensic Engineering Research Center, Department of Forensic Medicine, Hainan Medical University, Hainan Provincial Academician Workstation (Tropical Forensic Medicine).
  • Cai J; Hainan Vocational College of Political Science and Law, Hainan Gongping Forensic Center.
  • Cong B; Hainan Province Tropical Forensic Engineering Research Center, Department of Forensic Medicine, Hainan Medical University, Hainan Provincial Academician Workstation (Tropical Forensic Medicine); Department of Forensic Medicine, Hebei Medical University; hbydbincong@126.com.
  • Chen JH; Hainan Province Tropical Forensic Engineering Research Center, Department of Forensic Medicine, Hainan Medical University, Hainan Provincial Academician Workstation (Tropical Forensic Medicine); cjhtin86591@163.com.
  • Deng JQ; Hainan Province Tropical Forensic Engineering Research Center, Department of Forensic Medicine, Hainan Medical University, Hainan Provincial Academician Workstation (Tropical Forensic Medicine); forensicpatho@163.com.
J Vis Exp ; (207)2024 May 03.
Article en En | MEDLINE | ID: mdl-38767369
ABSTRACT
A total of five samples of Chrysomya megacephala samples - three fresh samples, one sample stored in alcohol for 2 years, and one sample stored in dry sealed storage for 2 years protected from light only - were selected to investigate whether a blood DNA extraction kit could extract DNA from necrophilous flies and to determine whether alcohol could prolong the preservation of necrophilous flies' DNA. First, the blood DNA extraction kit was used to extract DNA from their thorax tissues. Then, the DNA purity and concentration were examined using a microplate reader and a fluorometer. Finally, PCR amplification and electrophoresis of the extracted DNA were done with necrophilic fly-specific primers located in the mitochondrial CO I gene sequence. The results showed that the DNA purity of all samples was greater than 2.0. The DNA concentration was observed to be of the following order fresh samples > alcohol-preserved old samples > untreated, old samples. All samples had specific electrophoretic bands after PCR amplification. In conclusion, a blood DNA extraction kit can be used to extract DNA from necrophilic flies successfully, and the DNA concentration of fresh fly samples is greater than that of old fly samples. The flies can be stored in alcohol for a long time.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Reacción en Cadena de la Polimerasa Límite: Animals Idioma: En Revista: J Vis Exp Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Reacción en Cadena de la Polimerasa Límite: Animals Idioma: En Revista: J Vis Exp Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos