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Microfluidic Biochips for Single-Cell Isolation and Single-Cell Analysis of Multiomics and Exosomes.
Wang, Chao; Qiu, Jiaoyan; Liu, Mengqi; Wang, Yihe; Yu, Yang; Liu, Hong; Zhang, Yu; Han, Lin.
Afiliación
  • Wang C; Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China.
  • Qiu J; Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China.
  • Liu M; Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China.
  • Wang Y; Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China.
  • Yu Y; Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, 250100, China.
  • Liu H; State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China.
  • Zhang Y; Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China.
  • Han L; Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China.
Adv Sci (Weinh) ; 11(28): e2401263, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38767182
ABSTRACT
Single-cell multiomic and exosome analyses are potent tools in various fields, such as cancer research, immunology, neuroscience, microbiology, and drug development. They facilitate the in-depth exploration of biological systems, providing insights into disease mechanisms and aiding in treatment. Single-cell isolation, which is crucial for single-cell analysis, ensures reliable cell isolation and quality control for further downstream analyses. Microfluidic chips are small lightweight systems that facilitate efficient and high-throughput single-cell isolation and real-time single-cell analysis on- or off-chip. Therefore, most current single-cell isolation and analysis technologies are based on the single-cell microfluidic technology. This review offers comprehensive guidance to researchers across different fields on the selection of appropriate microfluidic chip technologies for single-cell isolation and analysis. This review describes the design principles, separation mechanisms, chip characteristics, and cellular effects of various microfluidic chips available for single-cell isolation. Moreover, this review highlights the implications of using this technology for subsequent analyses, including single-cell multiomic and exosome analyses. Finally, the current challenges and future prospects of microfluidic chip technology are outlined for multiplex single-cell isolation and multiomic and exosome analyses.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exosomas / Análisis de la Célula Individual Límite: Animals / Humans Idioma: En Revista: Adv Sci (Weinh) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exosomas / Análisis de la Célula Individual Límite: Animals / Humans Idioma: En Revista: Adv Sci (Weinh) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania