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Photoluminescence-based biosensor for the detection of antibodies against SARS-CoV-2 virus proteins by ZnO tetrapod structure integrated within microfluidic system.
Viter, Roman; Tepliakova, Iryna; Drobysh, Maryia; Zbolotnii, Viktor; Rackauskas, Simas; Ramanavicius, Simonas; Grundsteins, Karlis; Liustrovaite, Viktorija; Ramanaviciene, Almira; Ratautaite, Vilma; Brazys, Ernestas; Chen, Chien-Fu; Prentice, Urte; Ramanavicius, Arunas.
Afiliación
  • Viter R; Institute of Atomic Physics and Spectroscopy, University of Latvia, LV-1004 Riga, Latvia; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania. Electronic address: roman.viter@lu.lv.
  • Tepliakova I; Institute of Atomic Physics and Spectroscopy, University of Latvia, LV-1004 Riga, Latvia; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Drobysh M; State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Zbolotnii V; Institute of Atomic Physics and Spectroscopy, University of Latvia, LV-1004 Riga, Latvia; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Rackauskas S; Institute of Materials Science, Kaunas University of Technology, K. Barsausko St. 59, LT-51423 Kaunas, Lithuania.
  • Ramanavicius S; Institute of Atomic Physics and Spectroscopy, University of Latvia, LV-1004 Riga, Latvia; State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania.
  • Grundsteins K; Institute of Atomic Physics and Spectroscopy, University of Latvia, LV-1004 Riga, Latvia; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Liustrovaite V; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Ramanaviciene A; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Ratautaite V; State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Brazys E; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania.
  • Chen CF; Institute of Applied Mechanics, National Taiwan University, Taipei City 106, Taiwan.
  • Prentice U; State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania.
  • Ramanavicius A; State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania; NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania. Electronic address: arunas.ramanavicius@chf.vu.lt.
Sci Total Environ ; 939: 173333, 2024 Aug 20.
Article en En | MEDLINE | ID: mdl-38763199
ABSTRACT
This paper reports on development of an optical biosensor for the detection of antibodies against SARS-CoV-2 virus proteins in blood serum. ZnO nanotetrapods with high surface area and stable room temperature photoluminescence (PL) were selected as transducers. Structure and optical properties of the ZnO tetrapods have been studied by XRD, SEM and Raman spectroscopy. Crystallinity, dimensions and emission peaks of the ZnO tetrapods were determined. The ZnO tetrapods were fixed on glass chip. Silanization of ZnO tetrapods surface resulted in forming of functional surface groups suitable for the immobilization of bioselective layer. Two types of recombinant proteins (rS and rN) have been used to form bioselective layer on the surface of the ZnO tetrapods. Flow through microfluidic system, integrated with optical system, has been used for the determination of antibodies against SARS-CoV-2 virus proteins present in blood samples. The SARS-CoV-2 probes, prepared in PBS solution, have been injected into the measurement chamber with a constant pumping speed. Steady-state photoluminescence spectra and photoluminescence kinetics have been studied before and after injection of the probes. The biosensor signal has been tested to anti-SARS-CoV-2 antibodies in the range of 0.001 nM-1 nM. Control measurements have been performed with blood serum of healthy person. ZnO-SARS-CoV-2-rS and ZnO-SARS-CoV-2-rN biosensors showed high stability and sensitivity to anti-SARS-CoV-2 antibodies in the range of 0.025-0.5 nM (LOD 0.01 nM) and 0.3-1 nM (LOD 0.3 nM), respectively. Gibbs free energy of interaction between ZnO/SARS-CoV-2-rS and ZnO/SARS-CoV-2-rN bioselective layers with anti-SARS-CoV-2 antibodies showed -35.5 and -21.4 kJ/mol, respectively. Average detection time of biosensor integrated within microfluidic system was 15-20 min. The detection time and pumping speed (50 µL/min) were optimized to make detection faster. The developed system and ZnO-SARS-CoV-2-rS nanostructures have good potential for detection of anti-SARS-CoV-2 antibodies from patient's probes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxido de Zinc / Técnicas Biosensibles / SARS-CoV-2 / Anticuerpos Antivirales Límite: Humans Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxido de Zinc / Técnicas Biosensibles / SARS-CoV-2 / Anticuerpos Antivirales Límite: Humans Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos