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Comparative microbiome analysis in cystic fibrosis and non-cystic fibrosis bronchiectasis.
Motta, Heryk; Reuwsaat, Júlia Catarina Vieira; Lopes, Fernanda Cortez; Viezzer, Graciele; Volpato, Fabiana Caroline Zempulski; Barth, Afonso Luís; de Tarso Roth Dalcin, Paulo; Staats, Charley Christian; Vainstein, Marilene Henning; Kmetzsch, Lívia.
Afiliación
  • Motta H; Laboratório de Biologia Molecular de Patógenos, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Reuwsaat JCV; Laboratório de Biologia Molecular de Patógenos, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Lopes FC; Departamento de Biofísica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Viezzer G; Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Volpato FCZ; Serviço de Pneumologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • Barth AL; Laboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • de Tarso Roth Dalcin P; Laboratório de Pesquisa em Resistência Bacteriana, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • Staats CC; Serviço de Pneumologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • Vainstein MH; Departamento de Medicina Interna, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Kmetzsch L; Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Respir Res ; 25(1): 211, 2024 May 18.
Article en En | MEDLINE | ID: mdl-38762736
ABSTRACT

BACKGROUND:

Bronchiectasis is a condition characterized by abnormal and irreversible bronchial dilation resulting from lung tissue damage and can be categorized into two main groups cystic fibrosis (CF) and non-CF bronchiectasis (NCFB). Both diseases are marked by recurrent infections, inflammatory exacerbations, and lung damage. Given that infections are the primary drivers of disease progression, characterization of the respiratory microbiome can shed light on compositional alterations and susceptibility to antimicrobial drugs in these cases compared to healthy individuals.

METHODS:

To assess the microbiota in the two studied diseases, 35 subjects were recruited, comprising 10 NCFB and 13 CF patients and 12 healthy individuals. Nasopharyngeal swabs and induced sputum were collected, and total DNA was extracted. The DNA was then sequenced by the shotgun method and evaluated using the SqueezeMeta pipeline and R.

RESULTS:

We observed reduced species diversity in both disease cohorts, along with distinct microbial compositions and profiles of antimicrobial resistance genes, compared to healthy individuals. The nasopharynx exhibited a consistent microbiota composition across all cohorts. Enrichment of members of the Burkholderiaceae family and an increased Firmicutes/Bacteroidetes ratio in the CF cohort emerged as key distinguishing factors compared to NCFB group. Staphylococcus aureus and Prevotella shahii also presented differential abundance in the CF and NCFB cohorts, respectively, in the lower respiratory tract. Considering antimicrobial resistance, a high number of genes related to antibiotic efflux were detected in both disease groups, which correlated with the patient's clinical data.

CONCLUSIONS:

Bronchiectasis is associated with reduced microbial diversity and a shift in microbial and resistome composition compared to healthy subjects. Despite some similarities, CF and NCFB present significant differences in microbiome composition and antimicrobial resistance profiles, suggesting the need for customized management strategies for each disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bronquiectasia / Fibrosis Quística / Microbiota Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Respir Res Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bronquiectasia / Fibrosis Quística / Microbiota Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Respir Res Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido