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SIRT5 exacerbates eosinophilic chronic rhinosinusitis by promoting polarization of M2 macrophage.
Cha, Xu-Dong; Zou, Qing-Yun; Li, Feng-Zhen; Wang, Tian-Yu; Wang, Sheng-Lei; Cai, Bo-Yu; Cao, Zhi-Wen; Ji, Zhen-Hua; Liu, Hai-Bin; Wang, Wen-Wen; Li, Teng-Fei; Liang, Cai-Quan; Ren, Wen-Wen; Liu, Huan-Hai.
Afiliación
  • Cha XD; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Zou QY; Department of Otolaryngology, Naval Medical Center, Naval Medical University, Shanghai, China.
  • Li FZ; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Wang TY; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Wang SL; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Cai BY; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Cao ZW; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Ji ZH; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Liu HB; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Wang WW; Department of Neurology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Li TF; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China.
  • Liang CQ; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China. Electronic address: liangcq1993@126.com.
  • Ren WW; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China. Electronic address: wenwenren@smmu.edu.cn.
  • Liu HH; Department of Otolaryngology, the Second Affiliated Hospital of the Naval Medical University (Shanghai Changzheng Hospital), Shanghai, China. Electronic address: liuhuanhaiok@sina.com.
J Allergy Clin Immunol ; 154(3): 644-656, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38761998
ABSTRACT

BACKGROUND:

Previous studies implied that local M2 polarization of macrophage promoted mucosal edema and exacerbated TH2 type inflammation in chronic rhinosinusitis with nasal polyps (CRSwNP). However, the specific pathogenic role of M2 macrophages and the intrinsic regulators in the development of CRS remains elusive.

OBJECTIVE:

We sought to investigate the regulatory role of SIRT5 in the polarization of M2 macrophages and its potential contribution to the development of CRSwNP.

METHODS:

Real-time reverse transcription-quantitative PCR and Western blot analyses were performed to examine the expression levels of SIRT5 and markers of M2 macrophages in sinonasal mucosa samples obtained from both CRS and control groups. Wild-type and Sirt5-knockout mice were used to establish a nasal polyp model with TH2 inflammation and to investigate the effects of SIRT5 in macrophage on disease development. Furthermore, in vitro experiments were conducted to elucidate the regulatory role of SIRT5 in polarization of M2 macrophages.

RESULTS:

Clinical investigations showed that SIRT5 was highly expressed and positively correlated with M2 macrophage markers in eosinophilic polyps. The expression of SIRT5 in M2 macrophages was found to contribute to the development of the disease, which was impaired in Sirt5-deficient mice. Mechanistically, SIRT5 was shown to enhance the alternative polarization of macrophages by promoting glutaminolysis.

CONCLUSIONS:

SIRT5 plays a crucial role in promoting the development of CRSwNP by supporting alternative polarization of macrophages, thus providing a potential target for CRSwNP interventions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinusitis / Rinitis / Pólipos Nasales / Ratones Noqueados / Sirtuinas / Macrófagos Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: J Allergy Clin Immunol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinusitis / Rinitis / Pólipos Nasales / Ratones Noqueados / Sirtuinas / Macrófagos Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: J Allergy Clin Immunol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos