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Effect of pregnancy on isolation efficiency and in vitro proliferation of equine peripheral-blood derived mesenchymal stromal cells.
Mattei, Debora N; Harman, Rebecca M; Van de Walle, Gerlinde R; Smith, Roger; Grivel, Jean Charles; Abdelalim, Essam M; Vinardell, Tatiana.
Afiliación
  • Mattei DN; College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Member of Qatar Foundation, PO Box 34110, Education City, Doha, Qatar; Equine Veterinary Medical Center, Member of Qatar Foundation, P.O. Box 5825, Doha, Qatar.
  • Harman RM; Department of Microbiology and Immunology, Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Rd, Ithaca, NY 14850, USA.
  • Van de Walle GR; Department of Microbiology and Immunology, Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Rd, Ithaca, NY 14850, USA.
  • Smith R; Department of Clinical Science and Services, The Royal Veterinary College, Hawkshead Lane, Hatfield, Hertfordshire, AL9 7TA, United Kingdom.
  • Grivel JC; Deep Phenotyping Core, Sidra Medicine, PO Box 26999, Al Garrafa St, Ar-Rayyan, Doha, Qatar.
  • Abdelalim EM; College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Member of Qatar Foundation, PO Box 34110, Education City, Doha, Qatar; Laboratory of Pluripotent Stem Cell Disease Modeling, Translational Medicine Division, Research Branch, Sidra Medicine, P.O. Box 26999, Doha, Qatar; Diabet
  • Vinardell T; College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Member of Qatar Foundation, PO Box 34110, Education City, Doha, Qatar; Equine Veterinary Medical Center, Member of Qatar Foundation, P.O. Box 5825, Doha, Qatar. Electronic address: tatiana.vinardell@equinecaregroup.com.
Theriogenology ; 224: 107-118, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38761667
ABSTRACT
Mesenchymal stromal cells (MSCs) have regenerative and immunomodulatory potential and may be used to treat injured tissues. Pregnancy has been associated with increased MSCs in the peripheral circulation in multiple species, but to date, there are no reports on this matter in horses. This study aimed to evaluate the effect of pregnancy on isolation efficiency and proliferation capacity of equine MSCs derived from the peripheral blood (PB) of mares. Venous blood samples were collected at the 11th month of gestation and 1 month after delivery from clinically healthy Arabian mares that presented normal pregnancies. Blood samples were processed for in vitro cellular culture and hormonal and metabolic profiles. MSCs were isolated and characterized by trilineage differentiation potential, immunophenotyping, analyzed by gene sequencing and proliferation assays. The isolation of peripheral blood mononuclear cells (PBMCs) of pregnant mares were associated with higher isolation efficiency and proliferative capacity of MSCs derived from peripheral blood (PB-MSCs) recovered pre-partum than those isolated post-partum. Although fetal gender, parity, 5α-reduced pregnanes, insulin, and cortisol were shown to affect cellular proliferation, individual factors and the small population studied must be considered. This study suggests that PB-MSCs from pregnant mares could be a valuable alternative source of MSCs for therapeutic purposes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proliferación Celular / Células Madre Mesenquimatosas Límite: Animals / Pregnancy Idioma: En Revista: Theriogenology Año: 2024 Tipo del documento: Article País de afiliación: Qatar Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proliferación Celular / Células Madre Mesenquimatosas Límite: Animals / Pregnancy Idioma: En Revista: Theriogenology Año: 2024 Tipo del documento: Article País de afiliación: Qatar Pais de publicación: Estados Unidos