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Exogenous CaCl2 reduces the oxidative cleavage of carotenoids in shredded carrots by targeting CAMTA4-mediated transcriptional repression of carotenoid degradation pathway.
Zhang, Jinglin; Zhang, Kai; You, Wanli; Ru, Xueyin; Xu, Zhisheng; Xu, Feng; Jin, Peng; Zheng, Yonghua; Cao, Shifeng.
Afiliación
  • Zhang J; College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.
  • Zhang K; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.
  • You W; College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.
  • Ru X; College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.
  • Xu Z; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.
  • Xu F; Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315211, Zhejiang, PR China.
  • Jin P; College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.
  • Zheng Y; College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China. Electronic address: zhengyh@njau.edu.cn.
  • Cao S; College of Biological and Environmental Sciences, Key Laboratory of Fruit and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Wanli University, Ningbo, 315100, PR China. Electronic address: shifengcao1@gmail.com.
Plant Physiol Biochem ; 212: 108732, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38761546
ABSTRACT
Carotenoid oxidative cleavage is a significant factor contributing to the color changes of shredded carrots and treatment with calcium chloride (CaCl2, 1% w/v) has been observed to alleviate the whitening symptom and color loss. However, the specific mechanism by which CaCl2 treatment suppresses carotenoid degradation remains unclear. In this study, the effect of CaCl2 and EGTA (calcium ion chelating agent) treatment on carotenoid biosynthesis and degradation in shredded carrots and the mechanism involved was investigated. CaCl2 treatment promoted the expression and activity of carotenoid biosynthetic enzyme (phytoene synthase, PSY), but inhibited the increases of the degradative enzyme activity of carotenoid cleavage dioxygenase (CCD) and down-regulated the corresponding transcripts, thus delayed the degradation of total carotenoid and maintaining higher levels of major carotenoid compounds including ß-carotene, α-carotene, lycopene, and lutein in shredded carrots during storage. However, EGTA treatment promoted the gene expression and enzyme activity of CCD and increased the degradation of carotenoid compounds in shredded carrots during storage. Furthermore, the CaCl2 treatment induced DcCAMTA4, identified as a calcium decoder in shredded carrots, which, in turn, suppressed the expressions of DcCCD1 and DcCCD4 by interacting with their promoters. The transient overexpression of DcCAMTA4 in tobacco leaves led to reduced expression of NtCCD1 and NtCCD4, maintaining a higher content of carotenoids. Thus, CaCl2 alleviated the oxidative cleavage of carotenoids in shredded carrots through the DcCAMTA4-mediated carotenoid degradation pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Cloruro de Calcio / Carotenoides / Daucus carota Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2024 Tipo del documento: Article Pais de publicación: Francia
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Cloruro de Calcio / Carotenoides / Daucus carota Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2024 Tipo del documento: Article Pais de publicación: Francia