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A new methodology for a rapid and high-throughput comparison of molecular profiles and biological activity of phytoextracts.
Caldeira, Viviana; Fonseca, Tiago A H; N'Dembo, Luana; Araújo, Rúben; Von Rekowski, Cristiana P; Sampaio, Pedro N S; Calado, Cecília R C.
Afiliación
  • Caldeira V; ISEL - Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, Lisboa, Portugal.
  • Fonseca TAH; ISEL - Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, Lisboa, Portugal.
  • N'Dembo L; COPELABS-Computação e Cognição Centrada nas Pessoas, Faculty of Engineering, Lusófona University, Lisbon, Portugal.
  • Araújo R; ISEL - Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, Lisboa, Portugal.
  • Von Rekowski CP; ISEL - Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, Lisboa, Portugal.
  • Sampaio PNS; COPELABS-Computação e Cognição Centrada nas Pessoas, Faculty of Engineering, Lusófona University, Lisbon, Portugal.
  • Calado CRC; ISEL - Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa, Lisboa, Portugal.
Biotechnol Bioeng ; 2024 May 17.
Article en En | MEDLINE | ID: mdl-38760962
ABSTRACT
To robustly discover and explore phytocompounds, it is necessary to evaluate the interrelationships between the plant species, plant tissue, and the extraction process on the extract composition and to predict its cytotoxicity. The present work evaluated how Fourier Transform InfraRed spectroscopy can acquire the molecular profile of aqueous and ethanol-based extracts obtained from leaves, seeds, and flowers of Cynara Cardunculus, and ethanol-based extracts from Matricaria chamomilla flowers, as well the impact of these extracts on the viability of mammalian cells. The extract molecular profile enabled to predict the extraction yield, and how the plant species, plant tissue, and extraction process affected the extract's relative composition. The molecular profile obtained from the culture media of cells exposed to extracts enabled to capture its impact on cells metabolism, at a higher sensitivity than the conventional assay used to determine the cell viability. Furthermore, it was possible to detect specific impacts on the cell's metabolism according to plant species, plant tissue, and extraction process. Since spectra were acquired on small volumes of samples (25 µL), after a simple dehydration step, and based on a plate with 96 wells, the method can be applied in a rapid, simple, high-throughput, and economic mode, consequently promoting the discovery of phytocompounds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biotechnol Bioeng Año: 2024 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biotechnol Bioeng Año: 2024 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Estados Unidos