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In Vivo Modeling of Cryptococcus neoformans Infection and Collection of Murine Samples.
Muselius, Ben; Geddes-McAlister, Jennifer.
Afiliación
  • Muselius B; Molecular and Cellular Biology Department, University of Guelph, Guelph, ON, Canada.
  • Geddes-McAlister J; Molecular and Cellular Biology Department, University of Guelph, Guelph, ON, Canada. jgeddesm@uoguelph.ca.
Methods Mol Biol ; 2775: 47-55, 2024.
Article en En | MEDLINE | ID: mdl-38758310
ABSTRACT
In vivo models provide advantages to study the progression of disease and to identify potential biomarkers to detect and monitor infections. For the human fungal pathogen Cryptococcus neoformans, murine intranasal models aim to recapitulate natural infection from inhalation of desiccated fungal cells from the environment and permit monitoring of disease over time. In this chapter, we describe the establishment of a murine model for cryptococcosis and the subsequent collection of organs, tissues, and fluids for sampling. These samples may support novel diagnostic strategies and opportunities to monitor dissemination of the fungal cells throughout the host and propose new treatment options to combat disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criptococosis / Cryptococcus neoformans / Modelos Animales de Enfermedad Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criptococosis / Cryptococcus neoformans / Modelos Animales de Enfermedad Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos