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Biotin labeling allows for post-transfusion functional assessment of stored human platelets in mice.
Bailey, S Lawrence; Bochenek, Martin; Chauhan, Aastha; Miller, Brandon; Stolla, Moritz.
Afiliación
  • Bailey SL; Bloodworks Northwest Research Institute, Seattle, Washington, USA.
  • Bochenek M; Bloodworks Northwest Research Institute, Seattle, Washington, USA.
  • Chauhan A; Bloodworks Northwest Research Institute, Seattle, Washington, USA.
  • Miller B; Bloodworks Northwest Research Institute, Seattle, Washington, USA.
  • Stolla M; Bloodworks Northwest Research Institute, Seattle, Washington, USA.
Transfusion ; 64(7): 1306-1314, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38757806
ABSTRACT

BACKGROUND:

Platelet radiolabeling with radioisotopes is currently used for human platelet recovery and survival studies. Biotinylation enables ex vivo post-transfusion platelet function testing. Whether platelet biotinylation itself affects platelet function is controversial. STUDY DESIGN AND

METHODS:

Platelet concentrates from healthy humans were stored for 6 days. Samples were obtained at 1 or 2 and 6 days, and platelets were labeled following a radiolabeling protocol using saline instead of radioactive indium-111 (sham radiolabeling [sham-RL]). Alternatively, a newly developed biotinylation protocol, a washing protocol, or an unmanipulated control sample were used. Platelet function was assessed by flow cytometry after stimulation with platelet agonists and labeling of platelets with platelet activation markers. To test whether platelets can be activated after transfusion, labeled platelets were transfused into nonobese diabetic/severe combined immunodeficiency mice, and samples were obtained 1 h after transfusion.

RESULTS:

The activation profile of biotinylated platelets was comparable to sham-RL platelets before transfusion except for significantly less α-degranulation and more phosphatidyl serine exposure on storage day 1/2. There was no significant difference between sham-RL and biotinylated platelets on storage day 6. Sham-RL and biotinylated platelets were significantly less activatable than washed and unmanipulated control platelets. After transfusion, the activation profile of biotinylated platelets was largely indistinguishable from unmanipulated ones.

DISCUSSION:

The decrease in activation level in biotinylated platelets we and others observed appears mainly due to the physical manipulation during the labeling process. In conclusion, biotinylated platelets allow for post-transfusion function assessment, a major advantage over radiolabeling.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaquetas / Conservación de la Sangre / Ratones SCID / Transfusión de Plaquetas / Biotinilación Límite: Animals / Humans Idioma: En Revista: Transfusion Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaquetas / Conservación de la Sangre / Ratones SCID / Transfusión de Plaquetas / Biotinilación Límite: Animals / Humans Idioma: En Revista: Transfusion Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos