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Quantitative RUBY reporter assay for gene regulation analysis.
Liu, Jia; Li, Hao; Hong, Conghao; Lu, Wanqing; Zhang, Wei; Gao, Hongbo.
Afiliación
  • Liu J; National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
  • Li H; National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
  • Hong C; National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
  • Lu W; National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
  • Zhang W; National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
  • Gao H; National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Plant Cell Environ ; 2024 May 17.
Article en En | MEDLINE | ID: mdl-38757792
ABSTRACT
Various reporter genes have been developed to study gene expression pattern and gene regulation. The RUBY reporter gene was recently developed and widely used, because of its visible and noninvasive advantages. However, quantitative analysis of RUBY gene expression levels was lacking. In this study, we introduce a novel betalain quantification method in combination with the tobacco transient expression system. The betalain produced in tobacco leaves was extracted and purified, and its concentration was quantitatively measured. We successfully applied this approach in studying the transcriptional regulation of ARC5 gene by transcription factors CPD25 and CPD45. Furthermore, with this method, we showed that the gene expression of RCA and Rbcs1A gene were regulated by light, transcription factors HY5 and PIFs through G-box and I-box elements. The development of this betalain quantification approach with the tobacco transient expression system offers a cost-effective and intuitive strategy for studying the regulatory mechanism of gene expression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Environ Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Cell Environ Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos