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A NAC transcription factor represses a module associated with xyloglucan content and regulates aluminum tolerance.
Li, Su; Yang, Ji Bo; Li, Jia Qi; Huang, Jing; Shen, Ren Fang; Zeng, Da Li; Zhu, Xiao Fang.
Afiliación
  • Li S; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
  • Yang JB; Institute of Soil Science, University of Chinese Academy of Sciences, Beijing 100049, China.
  • Li JQ; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
  • Huang J; College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou 311300, China.
  • Shen RF; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
  • Zeng DL; Institute of Soil Science, University of Chinese Academy of Sciences, Beijing 100049, China.
  • Zhu XF; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Plant Physiol ; 196(1): 564-578, 2024 Sep 02.
Article en En | MEDLINE | ID: mdl-38753299
ABSTRACT
The transcriptional regulation of aluminum (Al) tolerance in plants is largely unknown, although Al toxicity restricts agricultural yields in acidic soils. Here, we identified a NAM, ATAF1/2, and cup-shaped cotyledon 2 (NAC) transcription factor that participates in Al tolerance in Arabidopsis (Arabidopsis thaliana). Al substantially induced the transcript and protein levels of ANAC070, and loss-of-function mutants showed remarkably increased Al sensitivity, implying a beneficial role of ANAC070 in plant tolerance to Al toxicity. Further investigation revealed that more Al accumulated in the roots of anac070 mutants, especially in root cell walls, accompanied by a higher hemicellulose and xyloglucan level, implying a possible interaction between ANAC070 and genes that encode proteins responsible for the modification of xyloglucan, including xyloglucan endo-transglycosylase/hydrolase (XTH) or ANAC017. Yeast 1-hybrid analysis revealed a potential interaction between ANAC070 and ANAC017, but not for other XTHs. Furthermore, dual-luciferase reporter assay, RT-qPCR, and GUS analysis revealed that ANAC070 could directly repress the transcript levels of ANAC017, and knockout of ANAC017 in the anac070 mutant partially restored its Al sensitivity phenotype, indicating that ANAC070 contributes to Al tolerance mechanisms other than suppression of ANAC017 expression. Further analysis revealed that the core transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) and its target genes, which control Al tolerance in Arabidopsis, may also be involved in ANAC070-regulated Al tolerance. In summary, we identified a transcription factor, ANAC070, that represses the ANAC017-XTH31 module to regulate Al tolerance in Arabidopsis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Xilanos / Arabidopsis / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Aluminio / Glucanos Idioma: En Revista: Plant Physiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Xilanos / Arabidopsis / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Aluminio / Glucanos Idioma: En Revista: Plant Physiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos