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Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets.
Dumas, Simon; Alexandre, Lucile; Richerd, Mathilde; Serra, Marco; Descroix, Stéphanie.
Afiliación
  • Dumas S; Institut Curie, Laboratoire Physico-Chimie (CNRS UMR 168), Institut Pierre-Gilles de Gennes, Sorbonne Université, PSL Research University, Paris, France.
  • Alexandre L; Institut Curie, Laboratoire Physico-Chimie (CNRS UMR 168), Institut Pierre-Gilles de Gennes, Sorbonne Université, PSL Research University, Paris, France.
  • Richerd M; Institut Curie, Laboratoire Physico-Chimie (CNRS UMR 168), Institut Pierre-Gilles de Gennes, Sorbonne Université, PSL Research University, Paris, France.
  • Serra M; Institut Curie, Laboratoire Physico-Chimie (CNRS UMR 168), Institut Pierre-Gilles de Gennes, Sorbonne Université, PSL Research University, Paris, France.
  • Descroix S; Institut Curie, UMR 168, Institut Pierre-Gilles de Gennes, Paris, France. stephanie.descroix@curie.fr.
Methods Mol Biol ; 2804: 163-176, 2024.
Article en En | MEDLINE | ID: mdl-38753147
ABSTRACT
Multiomics studies at single-cell level require small volume manipulation, high throughput analysis, and multiplexed detection, characteristics that droplet microfluidics can tackle. However, the initial step of molecule bioseparation remains challenging. Here, we describe a unique magnetic device to trap and extract magnetic particles in sub-nanoliter droplets, for compartmentalisation of detection steps. Relying on electrodeposition of NiFe structures and microfluidic manipulation, the extraction of 1 µm diameter magnetic particles was achieved at high throughput (20 droplets per second) with an efficiency close to 100% in 450 pL droplets. The first demonstration of its adaptability to single-cell analysis is demonstrated with the extraction of mRNA. Using a purified nucleic acid solution, this unique magnetic configuration was able to reach a RNA extraction rate of 72%. This is the first demonstration of a physical separation in droplets at high throughput at single-cell scale.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Análisis de la Célula Individual Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Análisis de la Célula Individual Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos