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Breaking the Mucin Barrier: A New Affinity Chromatography-Mass Spectrometry Approach to Unveil Potential Cell Markers and Pathways Altered in Pseudomyxoma Peritonei.
Romero-Ruiz, Antonio; Granados-Rodríguez, Melissa; Bura, Florina I; Valenzuela-Molina, Francisca; Rufián-Andújar, Blanca; Martínez-López, Ana; Rodríguez-Ortiz, Lidia; Ortega-Salas, Rosa; Torres-Martínez, María; Moreno-Serrano, Ana; Castaño, Justo; Michán, Carmen; Alhama, José; Vázquez-Borrego, Mari C; Arjona-Sánchez, Álvaro.
Afiliación
  • Romero-Ruiz A; Department of Biochemistry and Molecular Biology, University of Córdoba, Córdoba, Spain. b72rorua@uco.es.
  • Granados-Rodríguez M; Maimonides Biomedical Research Institute of Córdoba, IMIBIC and University of Córdoba, Av. Menéndez Pidal, s/n, Córdoba, 14004, Spain. b72rorua@uco.es.
  • Bura FI; Department of Biochemistry and Molecular Biology, University of Córdoba, Córdoba, Spain.
  • Valenzuela-Molina F; Maimonides Biomedical Research Institute of Córdoba, IMIBIC and University of Córdoba, Av. Menéndez Pidal, s/n, Córdoba, 14004, Spain.
  • Rufián-Andújar B; Department of Biochemistry and Molecular Biology, University of Córdoba, Córdoba, Spain.
  • Martínez-López A; Maimonides Biomedical Research Institute of Córdoba, IMIBIC and University of Córdoba, Av. Menéndez Pidal, s/n, Córdoba, 14004, Spain.
  • Rodríguez-Ortiz L; Maimonides Biomedical Research Institute of Córdoba, IMIBIC and University of Córdoba, Av. Menéndez Pidal, s/n, Córdoba, 14004, Spain.
  • Ortega-Salas R; Surgical Oncology Unit, Surgery Department, Reina Sofía University Hospital, Córdoba, Spain.
  • Torres-Martínez M; Maimonides Biomedical Research Institute of Córdoba, IMIBIC and University of Córdoba, Av. Menéndez Pidal, s/n, Córdoba, 14004, Spain.
  • Moreno-Serrano A; Surgical Oncology Unit, Surgery Department, Reina Sofía University Hospital, Córdoba, Spain.
  • Castaño J; Maimonides Biomedical Research Institute of Córdoba, IMIBIC and University of Córdoba, Av. Menéndez Pidal, s/n, Córdoba, 14004, Spain.
  • Michán C; Pathology Unit, HURS, Córdoba, Spain.
  • Alhama J; Maimonides Biomedical Research Institute of Córdoba, IMIBIC and University of Córdoba, Av. Menéndez Pidal, s/n, Córdoba, 14004, Spain.
  • Vázquez-Borrego MC; Surgical Oncology Unit, Surgery Department, Reina Sofía University Hospital, Córdoba, Spain.
  • Arjona-Sánchez Á; Maimonides Biomedical Research Institute of Córdoba, IMIBIC and University of Córdoba, Av. Menéndez Pidal, s/n, Córdoba, 14004, Spain.
Biol Proced Online ; 26(1): 13, 2024 May 15.
Article en En | MEDLINE | ID: mdl-38750435
ABSTRACT

BACKGROUND:

Pseudomyxoma peritonei (PMP) is a rare peritoneal mucinous carcinomatosis with largely unknown underlying molecular mechanisms. Cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy is the only therapeutic option; however, despite its use, recurrence with a fatal outcome is common. The lack of molecular characterisation of PMP and other mucinous tumours is mainly due to the physicochemical properties of mucin.

RESULTS:

This manuscript describes the first protocol capable of breaking the mucin barrier and isolating proteins from mucinous tumours. Briefly, mucinous tumour samples were homogenised and subjected to liquid chromatography using two specific columns to reduce mainly glycoproteins, albumins and immunoglobulin G. The protein fractions were then subjected to mass spectrometry analysis and the proteomic profile obtained was analysed using various bioinformatic tools. Thus, we present here the first proteome analysed in PMP and identified a distinct mucin isoform profile in soft compared to hard mucin tumour tissues as well as key biological processes/pathways altered in mucinous tumours. Importantly, this protocol also allowed us to identify MUC13 as a potential tumour cell marker in PMP.

CONCLUSIONS:

In sum, our results demonstrate that this protein isolation protocol from mucin will have a high impact, allowing the oncology research community to more rapidly advance in the knowledge of PMP and other mucinous neoplasms, as well as develop new and effective therapeutic strategies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biol Proced Online Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biol Proced Online Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido