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Regulation of Burkholderia cenocepacia virulence by the fatty acyl-CoA ligase DsfR as a response regulator of quorum sensing signal.
Li, Xia; Song, Shihao; Kong, Xiaohan; Chen, Xiayu; Zhao, Zhuoxian; Lin, Zizi; Jia, Yantao; Zhang, Yong; Luo, Hai-Bin; Wang, Qiao-Ping; Zhang, Lian-Hui; Qian, Wei; Deng, Yinyue.
Afiliación
  • Li X; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China.
  • Song S; Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou, China.
  • Kong X; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China.
  • Chen X; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China.
  • Zhao Z; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China.
  • Lin Z; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China.
  • Jia Y; State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
  • Zhang Y; College of Resources and Environment, Southwest University, Chongqing, China.
  • Luo HB; Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou, China.
  • Wang QP; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China.
  • Zhang LH; Integrative Microbiology Research Center, College of Plant Protection, South China Agricultural University, Guangzhou, China.
  • Qian W; State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
  • Deng Y; School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China. Electronic address: dengyle@mail.sysu.edu.cn.
Cell Rep ; 43(5): 114223, 2024 May 28.
Article en En | MEDLINE | ID: mdl-38748879
ABSTRACT
Quorum sensing (QS) is a cell-to-cell communication mechanism mediated by small diffusible signaling molecules. Previous studies showed that RpfR controls Burkholderia cenocepacia virulence as a cis-2-dodecenoic acid (BDSF) QS signal receptor. Here, we report that the fatty acyl-CoA ligase DsfR (BCAM2136), which efficiently catalyzes in vitro synthesis of lauryl-CoA and oleoyl-CoA from lauric acid and oleic acid, respectively, acts as a global transcriptional regulator to control B. cenocepacia virulence by sensing BDSF. We show that BDSF binds to DsfR with high affinity and enhances the binding of DsfR to the promoter DNA regions of target genes. Furthermore, we demonstrate that the homolog of DsfR in B. lata, RS02960, binds to the target gene promoter, and perception of BDSF enhances the binding activity of RS02960. Together, these results provide insights into the evolved unusual functions of DsfR that control bacterial virulence as a response regulator of QS signal.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Regulación Bacteriana de la Expresión Génica / Regiones Promotoras Genéticas / Coenzima A Ligasas / Percepción de Quorum / Burkholderia cenocepacia Límite: Animals Idioma: En Revista: Cell Rep Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Regulación Bacteriana de la Expresión Génica / Regiones Promotoras Genéticas / Coenzima A Ligasas / Percepción de Quorum / Burkholderia cenocepacia Límite: Animals Idioma: En Revista: Cell Rep Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos