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HYPOXIA induces lncRNA HOTAIR for recruiting RELA in papillary thyroid cancer cells to upregulate miR-181a and promote angiogenesis.
Lu, J; Liu, X; Cen, A; Hong, Y; Wang, Y.
Afiliación
  • Lu J; Department of Endocrinology, the First Affiliated Hospital of Jinan University, 613 W. Huangpu Avenue, Guangzhou, China.
  • Liu X; Department of Endocrinology, the First Affiliated Hospital of Jinan University, 613 W. Huangpu Avenue, Guangzhou, China.
  • Cen A; Department of Endocrinology, the People's Hospital of Jiangmen, Jiangmen, Guangdong, China.
  • Hong Y; Department of Endocrinology, Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, the Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
  • Wang Y; Department of Endocrinology, the First Affiliated Hospital of Jinan University, 613 W. Huangpu Avenue, Guangzhou, China. smeea@126.com.
J Endocrinol Invest ; 2024 May 15.
Article en En | MEDLINE | ID: mdl-38748197
ABSTRACT

BACKGROUND:

Papillary thyroid carcinoma (PTC) is one of the most common subtypes of thyroid carcinoma. Exosomal miR-181a plays an important role in the development of PTC. This study examined the regulatory mechanism of miR-181a under conditions of hypoxia and its impact on angiogenesis.

METHODS:

A ribonucleoprotein immunoprecipitation (RIP) experiment was conducted to verify the interaction between HOTAIR and RELA. The relationship between RELA and the miR-181a promoter was detected by ChIP-qPCR. Short hairpin (sh) RNA was designed to knock down HOTAIR in TPC cells. The underlying mechanism of miR-181a was verified by use of dual-luciferase assays and rescue experiments. The regulatory effect of GATA6 on angiogenesis was studied using CCK8, EdU, Transwell, and western blot assays.

RESULTS:

A RIP assay showed that HOTAIR could bind to RELA under hypoxic conditions. ChIP-qPCR and dual luciferase assays showed RELA could interact with the miR181a promoter and upregulate miR-181a. Knockdown of HOTAIR downregulated miR-181a in TPC-1 cells, and the downregulation could be rescued by RELA overexpression. MiR-181a downregulated GATA6 in HUVEC cells. Overexpression of GATA6 inhibited HUVEC proliferation, migration, tube formation, and EGFR expression. Exosomal miR-181a promoted angiogenesis by downregulating GATA6 expression.

CONCLUSION:

HOTAIR activated RELA to upregulate miR-181a during hypoxia. Exosomal miR-181a promotes tumor angiogenesis by downregulating GATA6.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Endocrinol Invest Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Italia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Endocrinol Invest Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Italia