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Evaluation of AAICare®-TB sequence analysis tool for accurate diagnosis of drug-resistant tuberculosis: A comparative study with TB-Profiler and Mykrobe.
Singhal, Ritu; Hingane, Smita; Bhalla, Manpreet; Sharma, Aniruddh; Ferdosh, Sehnaz; Tiwari, Avlokita; Jayaswal, Praapti; Yadav, Raj Narayan; Arora, Jyoti; Dewan, Ravindra Kumar; Sharma, Sangeeta.
Afiliación
  • Singhal R; Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, 110030, India. Electronic address: drritugo@gmail.com.
  • Hingane S; AarogyaAI® Innovations Pvt. Ltd., No. 677, 1st Floor, Suite 918, 13th Cross, Sector 1, HSR Layout, Bangalore, 560102, Karnataka, India.
  • Bhalla M; Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, 110030, India.
  • Sharma A; AarogyaAI® Innovations Pvt. Ltd., No. 677, 1st Floor, Suite 918, 13th Cross, Sector 1, HSR Layout, Bangalore, 560102, Karnataka, India.
  • Ferdosh S; AarogyaAI® Innovations Pvt. Ltd., No. 677, 1st Floor, Suite 918, 13th Cross, Sector 1, HSR Layout, Bangalore, 560102, Karnataka, India.
  • Tiwari A; AarogyaAI® Innovations Pvt. Ltd., No. 677, 1st Floor, Suite 918, 13th Cross, Sector 1, HSR Layout, Bangalore, 560102, Karnataka, India.
  • Jayaswal P; AarogyaAI® Innovations Pvt. Ltd., No. 677, 1st Floor, Suite 918, 13th Cross, Sector 1, HSR Layout, Bangalore, 560102, Karnataka, India.
  • Yadav RN; Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, 110030, India.
  • Arora J; Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, 110030, India.
  • Dewan RK; National Institute of Tuberculosis and Respiratory Diseases, New Delhi, 110030, India.
  • Sharma S; National Institute of Tuberculosis and Respiratory Diseases, New Delhi, 110030, India.
Tuberculosis (Edinb) ; 147: 102515, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38744006
ABSTRACT
A rapid and comprehensive drug susceptibility test is essential for eliminating drug resistant tuberculosis. Next generation sequencing (NGS) based susceptibility testing is being explored as a potential substitute for the conventional phenotypic and genotypic testing methods. However, the adoption of NGS based genotypic susceptibility testing depends on the availability of simple, accurate and efficient analysis tools. This preliminary study aimed to evaluate the performance of a Mycobacterium tuberculosis (Mtb) genome analysis pipeline, AAICare®-TB, for susceptibility prediction, in comparison to two widely used gDST prediction tools, TB-Profiler and Mykrobe. This study was performed in a National Reference Laboratory in India on presumptive drug-resistant tuberculosis (DR-TB) isolates. Whole genome sequences of the 120 cultured isolates were obtained through Illumina sequencing on a MiSeq platform. Raw sequences were simultaneously analysed using the three tools. Susceptibility prediction reports thus generated, were compared to estimate the total concordance and discordance. WHO mutation catalogue (1st edition, 2021) was used as the reference standard for categorizing the mutations. In this study, AAICare®-TB was able to predict drug resistance status for First Line (Streptomycin, Isoniazid, Rifampicin, Ethambutol and Pyrazinamide) and Second Line drugs (Fluoroquinolones, Second Line Injectables and Ethionamide) in 93 samples along with lineage and hetero-resistance as per the WHO guidelines.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Tuberculosis Resistente a Múltiples Medicamentos / Mycobacterium tuberculosis / Antituberculosos Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Tuberculosis (Edinb) Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Tuberculosis Resistente a Múltiples Medicamentos / Mycobacterium tuberculosis / Antituberculosos Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Tuberculosis (Edinb) Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido