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Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection.
Kaufman, Justin W; Singh, Brajesh K; Li, Ni; Sinn, Patrick L.
Afiliación
  • Kaufman JW; Stead Family Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA.
  • Singh BK; Stead Family Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA.
  • Li N; Scripps Research Institute, University of Florida, Jupiter, FL, USA.
  • Sinn PL; Stead Family Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA. patrick-sinn@uiowa.edu.
Methods Mol Biol ; 2808: 141-152, 2024.
Article en En | MEDLINE | ID: mdl-38743368
ABSTRACT
Measles virus (MeV) infection of airway surface epithelial cells provides a site for final amplification before being released back into the environment via coughing and sneezing. Multiple cell lines have served as models of polarized epithelia for MeV infection, such as Caco2 cells (intestinal derived human epithelia) or MDCK cells (kidney derived canine epithelia). In this chapter, we describe the materials and air-liquid interface (ALI) culture conditions for maintaining four different cell lines derived from human airway epithelial cells 16HBE14o-, Calu-3, H358, and NuLi-1. We provide methods for confirming transepithelial electrical resistance (TER) and preparing samples for microscopy as well as expected results from apical or basolateral MeV delivery. Polarized human airway derived cells serve as tissue culture models for investigating targeted questions about how MeV exits a human host. In addition, these methods are generalizable to studies of other respiratory viruses or the biology of ALI airway epithelial cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Células Epiteliales / Virus del Sarampión Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Célula / Células Epiteliales / Virus del Sarampión Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos