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Production of 1,4-Butanediol from Succinic Acid Using Escherichia Coli Whole-Cell Catalysis.
Ni, Ping; Gao, Cong; Wu, Jing; Song, Wei; Li, Xiaomin; Wei, Wanqing; Chen, Xiulai; Liu, Liming.
Afiliación
  • Ni P; School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, 214122.
  • Gao C; School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, 214122.
  • Wu J; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.
  • Song W; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.
  • Li X; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.
  • Wei W; School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, 214122.
  • Chen X; School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, 214122.
  • Liu L; School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, 214122.
Chembiochem ; 25(11): e202400142, 2024 Jun 03.
Article en En | MEDLINE | ID: mdl-38742957
ABSTRACT
The widespread attention towards 1,4-butanediol (BDO) as a key chemical raw material stems from its potential in producing biodegradable plastics. However, the efficiency of its biosynthesis via current bioprocesses is limited. In this study, a dual-pathway approach for 1,4-BDO production from succinic acid was developed. Specifically, a double-enzyme catalytic pathway involving carboxylic acid reductase and ethanol dehydrogenase was proposed. Optimization of the expression levels of the pathway enzymes led to a significant 318 % increase in 1,4-BDO titer. Additionally, the rate-limiting enzyme MmCAR was engineered to enhance the kcat/KM values by 50 % and increase 1,4-BDO titer by 46.7 %. To address cofactor supply limitations, an NADPH and ATP cycling system was established, resulting in a 48.9 % increase in 1,4-BDO production. Ultimately, after 48 hours, 1,4-BDO titers reached 201 mg/L and 1555 mg/L in shake flask and 5 L fermenter, respectively. This work represents a significant advancement in 1,4-BDO synthesis from succinic acid, with potential applications in the organic chemical and food industries.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Butileno Glicoles / Ácido Succínico / Escherichia coli Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Butileno Glicoles / Ácido Succínico / Escherichia coli Idioma: En Revista: Chembiochem Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Alemania