Comprehensive analysis of human monocyte subsets using full-spectrum flow cytometry and hierarchical marker clustering.
Front Immunol
; 15: 1405249, 2024.
Article
en En
| MEDLINE
| ID: mdl-38742110
ABSTRACT
Introduction:
Exploring monocytes' roles within the tumor microenvironment is crucial for crafting targeted cancer treatments.Methods:
This study unveils a novel methodology utilizing four 20-color flow cytometry panels for comprehensive peripheral immune system phenotyping, specifically targeting classical, intermediate, and non-classical monocyte subsets.Results:
By applying advanced dimensionality reduction techniques like t-distributed stochastic neighbor embedding (tSNE) and FlowSom analysis, we performed an extensive profiling of monocytes, assessing 50 unique cell surface markers related to a wide range of immunological functions, including activation, differentiation, and immune checkpoint regulation.Discussion:
This in-depth approach significantly refines the identification of monocyte subsets, directly supporting the development of personalized immunotherapies and enhancing diagnostic precision. Our pioneering panel for monocyte phenotyping marks a substantial leap in understanding monocyte biology, with profound implications for the accuracy of disease diagnostics and the success of checkpoint-inhibitor therapies. Key findings include revealing distinct marker expression patterns linked to tumor progression and providing new avenues for targeted therapeutic interventions.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Monocitos
/
Biomarcadores
/
Inmunofenotipificación
/
Citometría de Flujo
Límite:
Humans
Idioma:
En
Revista:
Front Immunol
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Suiza