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A study protocol to characterise pathophysiological and molecular markers of rheumatic heart disease and degenerative aortic stenosis using multiparametric cardiovascular imaging and multiomics techniques.
Mutithu, Daniel W; Aremu, Olukayode O; Mokaila, Dipolelo; Bana, Tasnim; Familusi, Mary; Taylor, Laura; Martin, Lorna J; Heathfield, Laura J; Kirwan, Jennifer A; Wiesner, Lubbe; Adeola, Henry A; Lumngwena, Evelyn N; Manganyi, Rodgers; Skatulla, Sebastian; Naidoo, Richard; Ntusi, Ntobeko A B.
Afiliación
  • Mutithu DW; Department of Medicine, Cape Heart Institute, University of Cape Town, Cape Town, South Africa.
  • Aremu OO; Division of Cardiology, Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa.
  • Mokaila D; Extramural Unit on Intersection of Noncommunicable Diseases and Infectious Diseases, South African Medical Research Council, Cape Town, South Africa.
  • Bana T; Department of Medicine, Cape Heart Institute, University of Cape Town, Cape Town, South Africa.
  • Familusi M; Division of Cardiology, Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa.
  • Taylor L; Extramural Unit on Intersection of Noncommunicable Diseases and Infectious Diseases, South African Medical Research Council, Cape Town, South Africa.
  • Martin LJ; Department of Medicine, Cape Heart Institute, University of Cape Town, Cape Town, South Africa.
  • Heathfield LJ; Division of Cardiology, Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa.
  • Kirwan JA; Extramural Unit on Intersection of Noncommunicable Diseases and Infectious Diseases, South African Medical Research Council, Cape Town, South Africa.
  • Wiesner L; Department of Medicine, Cape Heart Institute, University of Cape Town, Cape Town, South Africa.
  • Adeola HA; Division of Cardiology, Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa.
  • Lumngwena EN; Extramural Unit on Intersection of Noncommunicable Diseases and Infectious Diseases, South African Medical Research Council, Cape Town, South Africa.
  • Manganyi R; Division of Cardiology, Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa.
  • Skatulla S; Department of Civil Engineering, University of Cape Town, Cape Town, South Africa.
  • Naidoo R; Division of Forensic Medicine and Toxicology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
  • Ntusi NAB; Division of Forensic Medicine and Toxicology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
PLoS One ; 19(5): e0303496, 2024.
Article en En | MEDLINE | ID: mdl-38739622
ABSTRACT

INTRODUCTION:

Rheumatic heart disease (RHD), degenerative aortic stenosis (AS), and congenital valve diseases are prevalent in sub-Saharan Africa. Many knowledge gaps remain in understanding disease mechanisms, stratifying phenotypes, and prognostication. Therefore, we aimed to characterise patients through clinical profiling, imaging, histology, and molecular biomarkers to improve our understanding of the pathophysiology, diagnosis, and prognosis of RHD and AS.

METHODS:

In this cross-sectional, case-controlled study, we plan to recruit RHD and AS patients and compare them to matched controls. Living participants will undergo clinical assessment, echocardiography, CMR and blood sampling for circulatory biomarker analyses. Tissue samples will be obtained from patients undergoing valve replacement, while healthy tissues will be obtained from cadavers. Immunohistology, proteomics, metabolomics, and transcriptome analyses will be used to analyse circulatory- and tissue-specific biomarkers. Univariate and multivariate statistical analyses will be used for hypothesis testing and identification of important biomarkers. In summary, this study aims to delineate the pathophysiology of RHD and degenerative AS using multiparametric CMR imaging. In addition to discover novel biomarkers and explore the pathomechanisms associated with RHD and AS through high-throughput profiling of the tissue and blood proteome and metabolome and provide a proof of concept of the suitability of using cadaveric tissues as controls for cardiovascular disease studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estenosis de la Válvula Aórtica / Cardiopatía Reumática / Biomarcadores Límite: Female / Humans / Male Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Sudáfrica Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estenosis de la Válvula Aórtica / Cardiopatía Reumática / Biomarcadores Límite: Female / Humans / Male Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Sudáfrica Pais de publicación: Estados Unidos