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Manipulation of magnetic beads for actively capturing Vibrio parahaemolyticus and nucleic acid based on microfluidic system.
Zhang, Zhaoxuan; Deng, Xue; Zhang, Wenqiang; Chen, Kehan; Su, Yuan; Gao, Chao; Gong, De; Zhu, Longjiao; Cai, Jun.
Afiliación
  • Zhang Z; College of Engineering, China Agricultural University, Beijing 100083, China.
  • Deng X; College of Engineering, China Agricultural University, Beijing 100083, China.
  • Zhang W; College of Engineering, China Agricultural University, Beijing 100083, China.
  • Chen K; College of Engineering, China Agricultural University, Beijing 100083, China.
  • Su Y; Key Laboratory of Precision Nutrition and Quality Control of Food, Ministry of Education, Department of Nutrition and Health (Institute of Nutrition and Health), China Agricultural University, Beijing 100083, China.
  • Gao C; College of Engineering, China Agricultural University, Beijing 100083, China.
  • Gong; School of Mechanical Engineering and Automation, Beihang University, Beijing 100191, China.
  • Zhu L; Key Laboratory of Precision Nutrition and Quality Control of Food, Ministry of Education, Department of Nutrition and Health (Institute of Nutrition and Health), China Agricultural University, Beijing 100083, China.
  • Cai J; School of Mechanical Engineering and Automation, Beihang University, Beijing 100191, China.
Biomicrofluidics ; 18(3): 034104, 2024 May.
Article en En | MEDLINE | ID: mdl-38737753
ABSTRACT
Rapid biological detection of pathogen micro-organisms has attracted much attention for practical biomedical applications. Despite the development in this field, it is still challenging to achieve simple and rapid biological detection using the microfluidic method. Herein, we propose a novel strategy of biological detection that combines precise detection control of the capillary microfluidic chip and versatile manipulation of magnetic beads. The microfluidic chip was fabricated via laser cutting, which utilized capillary pressure to realize rapid passive injection of liquid samples. Under an external magnetic field, the aptamer-modified magnetic beads were actuated to mix with Vibrio parahaemolyticus (V. parahaemolyticus) and its nucleic acid in the capillary microfluidic chip for rapid selective capture and detection, which could be achieved within 40 min. The experimental results demonstrated that V. parahaemolyticus could be captured using on-chip immunomagnetic beads with a high efficiency and significantly enhanced detection value. Due to these superior performances, the capillary microfluidic system, based on the manipulation of magnetic beads, demonstrated great potential for automatic biological detection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomicrofluidics Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomicrofluidics Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos