Your browser doesn't support javascript.
loading
Possible crosstalk between the Arabidopsis TSPO-related protein and the transcription factor WRINKLED1.
Bhati, Kaushal Kumar; Luong, Ai My; Dittrich-Domergue, Franziska; D'Andrea, Sabine; Moreau, Patrick; Batoko, Henri.
Afiliación
  • Bhati KK; Louvain Institute of Biomolecular Science and Technology (LIBST), University of Louvain, Croix du Sud 4-5, L7.07.14, 1348, Louvain-la-Neuve, Belgium.
  • Luong AM; Louvain Institute of Biomolecular Science and Technology (LIBST), University of Louvain, Croix du Sud 4-5, L7.07.14, 1348, Louvain-la-Neuve, Belgium.
  • Dittrich-Domergue F; CNRS, University of Bordeaux, Laboratoire de Biogenèse Membranaire, UMR 5200, 33140, Villenave d'Ornon, France.
  • D'Andrea S; Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000, Versailles, France.
  • Moreau P; CNRS, University of Bordeaux, Laboratoire de Biogenèse Membranaire, UMR 5200, 33140, Villenave d'Ornon, France.
  • Batoko H; Louvain Institute of Biomolecular Science and Technology (LIBST), University of Louvain, Croix du Sud 4-5, L7.07.14, 1348, Louvain-la-Neuve, Belgium. Electronic address: henri.batoko@uclouvain.be.
Biochimie ; 224: 62-70, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38734125
ABSTRACT
This study uncovers a regulatory interplay between WRINKLED1 (WRI1), a master transcription factor for glycolysis and lipid biosynthesis, and Translocator Protein (TSPO) expression in Arabidopsis thaliana seeds. We identified potential WRI1-responsive elements upstream of AtTSPO through bioinformatics, suggesting WRI1's involvement in regulating TSPO expression. Our analyses showed a significant reduction in AtTSPO levels in wri1 mutant seeds compared to wild type, establishing a functional link between WRI1 and TSPO. This connection extends to the coordination of seed development and lipid metabolism, with both WRI1 and AtTSPO levels decreasing post-imbibition, indicating their roles in seed physiology. Further investigations into TSPO's impact on fatty acid synthesis revealed that TSPO misexpression alters WRI1's post-translational modifications and significantly enhances seed oil content. Additionally, we noted a decrease in key reserve proteins, including 12 S globulin and oleosin 1, in seeds with TSPO misexpression, suggesting a novel energy storage strategy in these lines. Our findings reveal a sophisticated network involving WRI1 and AtTSPO, highlighting their crucial contributions to seed development, lipid metabolism, and the modulation of energy storage mechanisms in Arabidopsis.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semillas / Factores de Transcripción / Arabidopsis / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis Idioma: En Revista: Biochimie Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Francia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semillas / Factores de Transcripción / Arabidopsis / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis Idioma: En Revista: Biochimie Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Francia