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Rapid colorimetric polymyxin B microelution directly from positive blood bottles: because patients with serious infections should not have to wait for results of culture-based methodologies.
Collar, Gabriela da Silva; Becker, Julia; Moreira, Natália Kehl; Dornelles, Luana Silva; Mott, Mariana Preussler; Barth, Afonso Luís; Caierão, Juliana.
Afiliación
  • Collar GDS; Laboratório de Pesquisa em Bacteriologia Clínica (LaBaC), Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. gabi.collar@gmail.com.
  • Becker J; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. gabi.collar@gmail.com.
  • Moreira NK; Laboratório de Pesquisa em Bacteriologia Clínica (LaBaC), Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Dornelles LS; Laboratório de Pesquisa em Bacteriologia Clínica (LaBaC), Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Mott MP; Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
  • Barth AL; Laboratório de Microbiologia Clínica, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
  • Caierão J; Laboratório de Microbiologia Clínica, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Eur J Clin Microbiol Infect Dis ; 43(7): 1407-1417, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38733425
ABSTRACT

PURPOSE:

To evaluate the performance of the rapid colorimetric polymyxin B microelution (RCPEm) in determining polymyxin B resistance directly from Enterobacterales-positive blood cultures.

METHODS:

A set volume of positive blood culture bottles (diluted 110) was inoculated into a glucose-broth-phenol red solution (NP solution), where a polymyxin B disk was previously eluted (final concentration of 3 µg/mL). Test was read each 1 h for up to 4 h. Color change from red/orange to yellow indicated resistant isolates. Results were compared to the reference method, broth microdilution (BMD), performed from colonies grown on solid media from the same blood culture bottle.

RESULTS:

One hundred fifty-two Enterobacterales-positive blood cultures were evaluated, 22.4% (34/152) of them resistant to polymyxin B (including 6.6% with borderline MICs). When performing directly from positive blood cultures (RCPEm-BC), specificity and sensitivity were 99.1% and 94.1%, respectively. Of note, 79.4% (27/34) of truly resistant isolates required 3 h of incubation, compared to the 18 ± 2 h incubation that microtiter plates of BMD demand before reading can be performed.

CONCLUSIONS:

RCPEm directly from blood cultures has great potential to be part of the routine of clinical microbiology laboratories to establish polymyxin B susceptibility, impacting outcome of patients with bloodstream infections caused by carbapenem-resistant Enterobacterales.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polimixina B / Pruebas de Sensibilidad Microbiana / Colorimetría / Cultivo de Sangre / Antibacterianos Límite: Humans Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polimixina B / Pruebas de Sensibilidad Microbiana / Colorimetría / Cultivo de Sangre / Antibacterianos Límite: Humans Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania