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Light-Emitting-Diode-Induced Fluorescence from Organic Dyes for Application in Excitation-Emission Fluorescence Spectroscopy for Food System Analysis.
Vladev, Veselin; Brazkova, Mariya; Bozhkov, Stefan; Angelova, Galena; Blazheva, Denica; Minkova, Stefka; Nikolova, Krastena; Eftimov, Tinko.
Afiliación
  • Vladev V; Department of Mathematics, Physics and Information Technologies, Faculty of Economics, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria.
  • Brazkova M; Central Laboratory of Applied Physics, Bulgarian Academy of Sciences, 61 Sankt Peterburg Blvd., 4002 Plovdiv, Bulgaria.
  • Bozhkov S; Department of Biotechnology, Technological Faculty, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria.
  • Angelova G; Department of Mathematics, Physics and Information Technologies, Faculty of Economics, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria.
  • Blazheva D; Department of Biotechnology, Technological Faculty, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria.
  • Minkova S; Department of Microbiology, Technological Faculty, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria.
  • Nikolova K; Department of Physics and Biophysics, Medical University-Varna, 84 Tzar Osvoboditel Blvd., 9000 Varna, Bulgaria.
  • Eftimov T; Department of Mathematics, Physics and Information Technologies, Faculty of Economics, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria.
Foods ; 13(9)2024 Apr 26.
Article en En | MEDLINE | ID: mdl-38731700
ABSTRACT
An experimental study is presented on the possibility of using the fluorescence from organic dyes as a broadband light source together with a monochromator for applications in excitation-emission matrix (EEM) fluorescence spectroscopy. A high-power single-chip light-emitting diode (LED) was chosen as an excitation source with a central output wavelength at 365 nm to excite a fluorescent solution of Coumarin 1 dye dissolved in ethanol. Two excitation configurations were investigated direct excitation from the LED and excitation through an optical-fiber-coupled LED. A Czerny-Turner monochromator with a diffraction grating was used for the spectral tuning of the fluorescence. A simple method was investigated for increasing the efficiency of the excitation as well as the fluorescence signal collection by using a diffuse reflector composed of barium sulfate (BaSO4) and polyvinyl alcohol (PVA). As research objects, extra-virgin olive oil (EVOO), Coumarin 6 dye, and Perylene, a polycyclic aromatic hydrocarbon (PAH), were used. The results showed that the light-emitting-diode-induced fluorescence was sufficient to cover the losses on the optical path to the monochromator output, where a detectable signal could be obtained. The obtained results reveal the practical possibility of applying the fluorescence from dyes as a light source for food system analysis by EEM fluorescence spectroscopy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Foods Año: 2024 Tipo del documento: Article País de afiliación: Bulgaria Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Foods Año: 2024 Tipo del documento: Article País de afiliación: Bulgaria Pais de publicación: Suiza