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Water-soluble vitamin-E for enhancing fluorescence diagnosis in infected human dentine treated with NaOCl.
Sin, Jonathan H; Walsh, Laurence J; Ranjit, Eliza; George, Roy.
Afiliación
  • Sin JH; School of Medicine and Dentistry, Griffith University, Gold Coast, Queensland, Australia.
  • Walsh LJ; School of Dentistry, The University of Queensland, Queensland, Australia.
  • Ranjit E; School of Medicine and Dentistry, Griffith University, Gold Coast, Queensland, Australia.
  • George R; School of Medicine and Dentistry, Griffith University, Gold Coast, Queensland, Australia. Electronic address: r.george@griffith.edu.au.
Photodiagnosis Photodyn Ther ; 47: 104208, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38729231
ABSTRACT

INTRODUCTION:

Bacterial fluorescence methods are of interest in endodontics for informing endpoints for debridement. This study explored potential fluorescence quenching reversal effects of a water-soluble vitamin E conjugate (d-α-Tocopherol polyethylene glycol 1000 succinate, TPGS) when applied to polymicrobial biofilms grown on dentine that had been exposed to sodium hypochlorite (NaOCl) to cause quenching.

METHOD:

Extracted human teeth were debrided, embedded in transparent acrylic resin and sectioned. After smear layer removal, tooth dentine sections were inoculated with a polymicrobial inoculum, and cultured for 7 days to create biofilms. Samples (n = 8 per group) were exposed to 1 % or 4 % NaOCl for 2 or 4 min, and then treated with TPGS. Bacterial fluorescence readings under laser excitation at 655 nm were assessed over 10 min using a calibrated DIAGNOdent device. All data were assessed for normality (Kolmogorov-Smirnov test) and analysed with ANOVA followed by Bonferroni post-hoc tests.

RESULTS:

NaOCl at both concentrations quenched fluorescence readings of biofilms grown on dentine samples, with a maximal reduction of 40.4 % at 5 min after 4 % NaOCl. Treatment with TPGS gave faster recovery of fluorescence readings compared to the control at 5 and 10 min.

CONCLUSION:

The water-soluble antioxidant TPGS partially reversed fluorescence quenching caused by NaOCl. This agent may have value clinically for reducing the time needed for fluorescence readings to recover when NaOCl is used as an irrigant. This will facilitate more accurate assessment of endpoints for canal debridement.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipoclorito de Sodio / Vitamina E / Biopelículas / Dentina Límite: Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Asunto de la revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipoclorito de Sodio / Vitamina E / Biopelículas / Dentina Límite: Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Asunto de la revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos