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Electrochemical Detection of a Breast Cancer Biomarker with an Amine-Functionalized Nanocomposite Pt-Fe3O4-MWCNTs-NH2 as a Signal-Amplifying Label.
Patil, Sunil M; Karade, Vijay C; Kim, Jin Hyeok; Chougale, Ashok D; Patil, Prashant B.
Afiliación
  • Patil SM; Department of Physics, The New College, Shivaji University, Kolhapur 416012, Maharashtra, India.
  • Karade VC; Optoelectronic Convergence Research Center and Department of Materials Science and Engineering, Chonnam National University, Gwangju 500-757, South Korea.
  • Kim JH; Optoelectronic Convergence Research Center and Department of Materials Science and Engineering, Chonnam National University, Gwangju 500-757, South Korea.
  • Chougale AD; Department of Chemistry, The New College, Shivaji University, Kolhapur 416012, Maharashtra, India.
  • Patil PB; Department of Physics, The New College, Shivaji University, Kolhapur 416012, Maharashtra, India.
ACS Appl Mater Interfaces ; 16(20): 25601-25609, 2024 May 22.
Article en En | MEDLINE | ID: mdl-38727578
ABSTRACT
We report an ultrasensitive sandwich-type electrochemical immunosensor to detect the breast cancer biomarker CA 15-3. Amine-functionalized composite of reduced graphene oxide and Fe3O4 nanoparticles (MRGO-NH2) was used as an electrochemical sensing platform material to modify the electrodes. The nanocomposite comprising Pt and Fe3O4 nanoparticles (NPs) anchored on multiwalled carbon nanotubes (Pt-Fe3O4-MWCNTs-NH2) was utilized as a pseudoenzymatic signal-amplifying label. Compared to reduced graphene oxide, the composite MRGO-NH2 platform material demonstrated a higher electrochemical signal. In the Pt-Fe3O4-MWCNTs-NH2 label, multiwalled carbon nanotubes provided the substratum to anchor abundant catalytic Pt and Fe3O4 NPs. The nanocomposites were thoroughly characterized using transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and X-ray photoelectron spectroscopy. An electroanalytical study and prevalidation of the immunosensor was carried out. The immunosensor exhibited exceptional capabilities in detecting CA 15-3, offering a wider linear range of 0.0005-100 U mL-1 and a lower detection limit of 0.00008 U mL-1. Moreover, the designed immunosensor showed good specificity, reproducibility, and acceptable stability. The sensor was successfully applied to analyze samples from breast cancer patients, yielding reliable results.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Platino (Metal) / Neoplasias de la Mama / Biomarcadores de Tumor / Nanotubos de Carbono / Nanocompuestos / Técnicas Electroquímicas Límite: Female / Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Platino (Metal) / Neoplasias de la Mama / Biomarcadores de Tumor / Nanotubos de Carbono / Nanocompuestos / Técnicas Electroquímicas Límite: Female / Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos