Analysis of microcystins in alum water treatment sludges: holding times, temperatures, linearity of response, and sensitivity to pre-coagulation cell titers.
Environ Technol
; : 1-14, 2024 May 09.
Article
en En
| MEDLINE
| ID: mdl-38723193
ABSTRACT
ELISA assays are a potential tool to screen for dissolved or cell bound microcystins in drinking water treatment sludges. In order to evaluate this potential more thoroughly, experiments were performed in alum sludges to (1) evaluate the impacts of sample storage times, temperatures, and sludge composition on spiked microcystin-LR recovery by ELISA; (2) examine the linearity of ELISA responses to spiked microcystin-LR as a function of sludge composition; and (3) examine the sensitivity ELISA and LC/MS/MS to five different concentrations of microcystin-producing cyanobacteria entrained in sludges of two different compositions. During storage experiments, microcystin recovery efficiencies ranged from 85% to 125% across the range of 12 storage time and temperature combinations with recovery efficiencies in 7 of the 12 combinations falling into the 90% to 110% range. During the linearity experiments, linear models fit ELISA responses in all sludge compositions with R2 values ≥ 0.95. During the sensitivity studies, simple freeze/thaw/centrifugation processing combined with ELISA or LC/MS/MS analyses resulted in detection of microcystins in thickened sludges derived from pre-coagulation cell suspensions of 102-106 cells/mL. In addition, the relationships between toxin concentrations in sludges and the original cell titers were linear regardless of analytical method.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Environ Technol
Asunto de la revista:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Reino Unido