Enhanced Herbicide Metabolism and Target Site Mutation Enabled the Multiple Resistance to Cyhalofop-butyl, Florpyrauxifen-benzyl, and Penoxsulam in <i>Echinochloa crus-galli</i>.

Yu, Xiaoyue; Sun, Jinqiu; Yang, Yongjie; Zhang, Jianping; Lu, Yongliang; Tang, Wei

Enhanced Herbicide Metabolism and Target Site Mutation Enabled the Multiple Resistance to Cyhalofop-butyl, Florpyrauxifen-benzyl, and Penoxsulam in Echinochloa crus-galli.

Yu, Xiaoyue; Sun, Jinqiu; Yang, Yongjie; Zhang, Jianping; Lu, Yongliang; Tang, Wei.

Afiliación

Yu X; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
Sun J; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
Yang Y; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
Zhang J; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
Lu Y; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.
Tang W; State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China.

J Agric Food Chem ; 72(20): 11405-11414, 2024 May 22.

Article en En | MEDLINE | ID: mdl-38717990

ABSTRACT

ABSTRACT

This study investigated the multiple herbicide resistance (MHR) mechanism of one Echinochloa crus-galli population that was resistant to florpyrauxifen-benzyl (FPB), cyhalofop-butyl (CHB), and penoxsulam (PEX). This population carried an Ala-122-Asn mutation in the acetolactate synthase (ALS) gene but no mutation in acetyl-CoA carboxylase (ACCase) and transport inhibitor response1 (TIR1) genes. The metabolism rate of PEX was 2-fold higher, and the production of florpyrauxifen-acid and cyhalofop-acid was lower in the resistant population. Malathion and 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) could reverse the resistance, suggesting that cytochrome P450 (CYP450) and glutathione S-transferase (GST) contribute to the enhanced metabolism. According to RNA-seq and qRT-PCR validation, two CYP450 genes (CYP71C42 and CYP71D55), one GST gene (GSTT2), two glycosyltransferase genes (rhamnosyltransferase 1 and IAAGLU), and two ABC transporter genes (ABCG1 and ABCG25) were induced by CHB, FPB, and PEX in the resistant population. This study revealed that the target mutant and enhanced metabolism were involved in the MHR mechanism in E. crus-galli.

Asunto(s)

Sistema Enzimático del Citocromo P-450; Echinochloa; Resistencia a los Herbicidas; Herbicidas; Mutación; Proteínas de Plantas; Resistencia a los Herbicidas/genética; Herbicidas/farmacología; Herbicidas/metabolismo; Echinochloa/genética; Echinochloa/efectos de los fármacos; Echinochloa/metabolismo; Echinochloa/crecimiento & desarrollo; Proteínas de Plantas/genética; Proteínas de Plantas/metabolismo; Sistema Enzimático del Citocromo P-450/genética; Sistema Enzimático del Citocromo P-450/metabolismo; Acetil-CoA Carboxilasa/genética; Acetil-CoA Carboxilasa/metabolismo; Malezas/efectos de los fármacos; Malezas/genética; Malezas/metabolismo; Acetolactato Sintasa/genética; Acetolactato Sintasa/metabolismo; Butanos; Nitrilos; Sulfonamidas; Uridina/análogos & derivados

Palabras clave

Echinochloa crus-galli; RNA-seq; enhanced herbicide metabolism; multiple herbicide resistance; target site mutation

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Sistema Enzimático del Citocromo P-450 / Echinochloa / Resistencia a los Herbicidas / Herbicidas / Mutación Idioma: En Revista: J Agric Food Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

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