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Molecular Basis of Cerebral Vasospasm: What Can We Learn from Transcriptome and Temporal Gene Expression Profiling in Intracranial Aneurysm?
Kumar, Munish; Sharma, Tanavi; Patel, Krishna; Chinnapparaj, Shobia; Dixit, Ravi; Gendle, Chandrashekhar; Aggarwal, Ashish; Takkar, Aastha; Gupta, Tulika; Singla, Navneet; Pal, Arnab; Salunke, Pravin; Dhandapani, Sivashanmugam; Chabra, Rajesh; Chatterjee, Aditi; Gowda, Harsha; Bhagat, Hemant.
Afiliación
  • Kumar M; Division of Neuro-anesthesia, Department of Anesthesia and Intensive Care, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Sharma T; Division of Neuro-anesthesia, Department of Anesthesia and Intensive Care, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Patel K; Institute of Bioinformatics, International Tech Park, Bangalore, India.
  • Chinnapparaj S; Division of Neuro-anesthesia, Department of Anesthesia and Intensive Care, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Dixit R; Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Gendle C; Department of Neurosurgery, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Aggarwal A; Department of Neurosurgery, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Takkar A; Department of Neurology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Gupta T; Department of Anatomy, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Singla N; Department of Neurosurgery, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Pal A; Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Salunke P; Department of Neurosurgery, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Dhandapani S; Department of Neurosurgery, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Chabra R; Department of Neurosurgery, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Chatterjee A; Institute of Bioinformatics, International Tech Park, Bangalore, India.
  • Gowda H; Institute of Bioinformatics, International Tech Park, Bangalore, India.
  • Bhagat H; Division of Neuro-anesthesia, Department of Anesthesia and Intensive Care, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
OMICS ; 28(5): 234-245, 2024 05.
Article en En | MEDLINE | ID: mdl-38717843
ABSTRACT
Cerebral vasospasm (CV) is a significant complication following aneurysmal subarachnoid hemorrhage (aSAH), and lacks a comprehensive molecular understanding. Given the temporal trajectory of intracranial aneurysm (IA) formation, its rupture, and development of CV, altered gene expression might be a molecular substrate that runs through these clinical events, influencing both disease inception and progression. Utilizing RNA-Seq, we analyzed tissue samples from ruptured IAs with and without vasospasm to identify the dysregulated genes. In addition, temporal gene expression analysis was conducted. We identified seven dysregulated genes in patients with ruptured IA with vasospasm when compared with those without vasospasm. We found 192 common genes when the samples of each clinical subset of patients with IA, that is, unruptured aneurysm, ruptured aneurysm without vasospasm, and ruptured aneurysm with vasospasm, were compared with control samples. Among these common genes, TNFSF13B, PLAUR, OSM, and LAMB3 displayed temporal expression (progressive increase) with the pathological progression of disease that is formation of aneurysm, its rupture, and consequently the development of vasospasm. We validated the temporal gene expression pattern of OSM at both the transcript and protein levels and OSM emerges as a crucial gene implicated in the pathological progression of disease. In addition, RSAD2 and ATP1A2 appear to be pivotal genes for CV development. To the best of our knowledge, this is the first study to compare the transcriptome of aneurysmal tissue samples of aSAH patients with and without CV. The findings collectively provide new insights on the molecular basis of IA and CV and new leads for translational research.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aneurisma Intracraneal / Vasoespasmo Intracraneal / Perfilación de la Expresión Génica / Transcriptoma Límite: Female / Humans / Male / Middle aged Idioma: En Revista: OMICS Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aneurisma Intracraneal / Vasoespasmo Intracraneal / Perfilación de la Expresión Génica / Transcriptoma Límite: Female / Humans / Male / Middle aged Idioma: En Revista: OMICS Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos