Your browser doesn't support javascript.
loading
Presence of phage-plasmids in multiple serovars of Salmonella enterica.
Nair, Satheesh; Barker, Clare R; Bird, Matthew; Greig, David R; Collins, Caitlin; Painset, Anaïs; Chattaway, Marie; Pickard, Derek; Larkin, Lesley; Gharbia, Saheer; Didelot, Xavier; Ribeca, Paolo.
Afiliación
  • Nair S; UK Health Security Agency, London, UK.
  • Barker CR; UK Health Security Agency, London, UK.
  • Bird M; NIHR Health Protection Research Unit in Genomics and Enabling Data, University of Warwick, Warwick, UK.
  • Greig DR; UK Health Security Agency, London, UK.
  • Collins C; NIHR Health Protection Research Unit in Healthcare Associated Infections and Antimicrobial Resistance, University of Oxford, Oxford, UK.
  • Painset A; UK Health Security Agency, London, UK.
  • Chattaway M; NIHR Health Protection Research Unit in Gastrointestinal Infections, University of Liverpool, Liverpool, UK.
  • Pickard D; Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK.
  • Larkin L; UK Health Security Agency, London, UK.
  • Gharbia S; NIHR Health Protection Research Unit in Genomics and Enabling Data, University of Warwick, Warwick, UK.
  • Didelot X; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
  • Ribeca P; UK Health Security Agency, London, UK.
Microb Genom ; 10(5)2024 May.
Article en En | MEDLINE | ID: mdl-38717818
ABSTRACT
Evidence is accumulating in the literature that the horizontal spread of antimicrobial resistance (AMR) genes mediated by bacteriophages and bacteriophage-like plasmid (phage-plasmid) elements is much more common than previously envisioned. For instance, we recently identified and characterized a circular P1-like phage-plasmid harbouring a bla CTX-M-15 gene conferring extended-spectrum beta-lactamase (ESBL) resistance in Salmonella enterica serovar Typhi. As the prevalence and epidemiological relevance of such mechanisms has never been systematically assessed in Enterobacterales, in this study we carried out a follow-up retrospective analysis of UK Salmonella isolates previously sequenced as part of routine surveillance protocols between 2016 and 2021. Using a high-throughput bioinformatics pipeline we screened 47 784 isolates for the presence of the P1 lytic replication gene repL, identifying 226 positive isolates from 25 serovars and demonstrating that phage-plasmid elements are more frequent than previously thought. The affinity for phage-plasmids appears highly serovar-dependent, with several serovars being more likely hosts than others; most of the positive isolates (170/226) belonged to S. Typhimurium ST34 and ST19. The phage-plasmids ranged between 85.8 and 98.2 kb in size, with an average length of 92.1 kb; detailed analysis indicated a high amount of diversity in gene content and genomic architecture. In total, 132 phage-plasmids had the p0111 plasmid replication type, and 94 the IncY type; phylogenetic analysis indicated that both horizontal and vertical gene transmission mechanisms are likely to be involved in phage-plasmid propagation. Finally, phage-plasmids were present in isolates that were resistant and non-resistant to antimicrobials. In addition to providing a first comprehensive view of the presence of phage-plasmids in Salmonella, our work highlights the need for a better surveillance and understanding of phage-plasmids as AMR carriers, especially through their characterization with long-read sequencing.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásmidos / Salmonella enterica / Serogrupo Límite: Humans Idioma: En Revista: Microb Genom Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásmidos / Salmonella enterica / Serogrupo Límite: Humans Idioma: En Revista: Microb Genom Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido