Phytochemical profiling, cytotoxic, anti-migration, and anti-angiogenic potential of phenolic-rich fraction from Peganum harmala: in vitro and in ovo studies.

Kemel, Hadjer; Benguedouar, Lamia; Boudjerda, Djamel; Menadi, Soumaya; Cacan, Ercan; Sifour, Mohamed

Kemel, Hadjer; Benguedouar, Lamia; Boudjerda, Djamel; Menadi, Soumaya; Cacan, Ercan; Sifour, Mohamed.

Afiliación

Kemel H; Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Jijel, 18000, Jijel, Algeria. hadjer.kemel@univ-jijel.dz.
Benguedouar L; Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Jijel, 18000, Jijel, Algeria.
Boudjerda D; Laboratory of Molecular and Cellular Biology, Faculty of Nature and Life Sciences, University of Jijel, 18000, Jijel, Algeria.
Menadi S; Department of Molecular Biology and Genetics, Tokat Gaziosmanpasa University, 60250, Tokat, Turkey.
Cacan E; Department of Molecular Biology and Genetics, Tokat Gaziosmanpasa University, 60250, Tokat, Turkey.
Sifour M; Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Jijel, 18000, Jijel, Algeria.

Med Oncol ; 41(6): 144, 2024 May 08.

Article en En | MEDLINE | ID: mdl-38717574

ABSTRACT

ABSTRACT

Peganum harmala has been extensively employed in Algerian traditional medicine practices. This study aimed to explore the impact of n-butanol (n-BuOH) extract sourced from Peganum harmala seeds on cell proliferation, cell migration, and angiogenesis inhibition. Cytotoxic potential of n-BuOH extract was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl) 2,5 diphenyltetrazolium bromide) assay against human breast adenocarcinoma MCF-7 cells, cell migration was determined using scratch assay, and anti-angiogenic effect was evaluated through macroscopic and histological examinations conducted on chick embryo chorioallantoic membrane. Additionally, this research estimated the phytochemical profile of n-BuOH extract. Fifteen phenolic compounds were identified using Ultra-performance liquid chromatography UPLC-ESI-MS-MS analysis. In addition, the n-BuOH extract of P. harmala exhibited potent antioxidant and free radical scavenging properties. The n-BuOH extract showed potent cytotoxicity against MCF-7 cell with an IC50 value of 8.68 ± 1.58 µg/mL. Furthermore, n-BuOH extract significantly reduced migration. A strong anti-angiogenic activity was observed in the groups treated with n-BuOH extract in comparison to the negative control. Histological analysis confirmed the anti-angiogenic effect of the n-BuOH extract. This activity is probably a result of the synergistic effects produced by different polyphenolic classes.

Asunto(s)

Inhibidores de la Angiogénesis; Movimiento Celular; Peganum; Fenoles; Extractos Vegetales; Humanos; Movimiento Celular/efectos de los fármacos; Extractos Vegetales/farmacología; Extractos Vegetales/química; Peganum/química; Embrión de Pollo; Fenoles/farmacología; Fenoles/análisis; Inhibidores de la Angiogénesis/farmacología; Células MCF-7; Animales; Proliferación Celular/efectos de los fármacos; Fitoquímicos/farmacología; Fitoquímicos/química; Antioxidantes/farmacología; Antioxidantes/química; Antineoplásicos Fitogénicos/farmacología; Membrana Corioalantoides/efectos de los fármacos; Membrana Corioalantoides/irrigación sanguínea

Palabras clave

Peganum harmala; Anti-angiogenic; Chorioallantoic membrane; MCF-7 cells; Migration

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenoles / Extractos Vegetales / Movimiento Celular / Inhibidores de la Angiogénesis / Peganum Límite: Animals / Humans Idioma: En Revista: Med Oncol Asunto de la revista: NEOPLASIAS Año: 2024 Tipo del documento: Article País de afiliación: Argelia Pais de publicación: Estados Unidos

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