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CXCR2/Snail-1-Induced Epithelial-Mesenchymal Transition in the Formation and Progression of RCC with Inferior Vena Cava Tumour Thrombus.
Wang, Lei; Gao, Jingyu; Zheng, Shuo; Luo, Zijing; Xu, Zhe; Che, Hang; Wang, Zhao.
Afiliación
  • Wang L; Department of Urology, The First Hospital of Hebei Medical University, 050031 Shijiazhuang, Hebei, China.
  • Gao J; Department of Urology, The First Hospital of Hebei Medical University, 050031 Shijiazhuang, Hebei, China.
  • Zheng S; Department of Urology, The First Hospital of Hebei Medical University, 050031 Shijiazhuang, Hebei, China.
  • Luo Z; Department of Urology, The First Hospital of Hebei Medical University, 050031 Shijiazhuang, Hebei, China.
  • Xu Z; Department of Urology, The First Hospital of Hebei Medical University, 050031 Shijiazhuang, Hebei, China.
  • Che H; Department of Urology, The First Hospital of Hebei Medical University, 050031 Shijiazhuang, Hebei, China.
  • Wang Z; Department of Urology, The First Hospital of Hebei Medical University, 050031 Shijiazhuang, Hebei, China.
Arch Esp Urol ; 77(3): 292-302, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38715171
ABSTRACT

BACKGROUND:

Renal cell carcinoma (RCC), a common and highly invasive malignant tumour, presents clinical challenges due to its propensity for easy metastasis. Inferior vena cava tumour thrombus is a common RCC complication significantly impacting patient prognosis. This study investigates C-X-C chemokine receptor type 2 (CXCR2)/Snail-1-induced epithelial-mesenchymal transition (EMT) in RCC with inferior vena cava tumour thrombus.

METHODS:

Tissues from 51 RCC patients were analysed for CXCR2 and Snail-1 Messenger Ribonucleic Acid (mRNA) levels using Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). Elevated levels of both were observed in tumour and inferior vena cava tumour thrombus tissues. Using Short Hairpin RNA (shRNA) technology, we inhibited CXCR2 and Snail-1 expression to investigate their impact on EMT, invasiveness, and metastatic potential in RCC cells.

RESULTS:

Compared with that in the Short Hairpin RNA-Negative Control (ShNC) group, inhibition of CXCR2 and Snail-1 suppressed the degree of EMT, invasiveness, and metastatic ability of RCC cells (p < 0.01). Further mechanistic studies showed that CXCR2/Snail-1 participated in the formation and progression of RCC by regulating the extracellular signal-regulated kinase 1/2 (ERK1/2) signalling pathways. Additionally, compared with that in the ShNC group, knockdown of CXCR2 and Snail-1 significantly inhibited the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9; p < 0.01), thereby regulating the metastasis of RCC.

CONCLUSIONS:

Our findings suggest that CXCR2/Snail-1-induced EMT plays an important role in the formation and progression of RCC with inferior vena cava tumour thrombus. CXCR2/Snail-1 participates in the invasion and metastasis of RCC by regulating the expression of multiple signalling pathways and related genes. These results provide new insights and directions for the treatment of RCC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vena Cava Inferior / Carcinoma de Células Renales / Progresión de la Enfermedad / Transición Epitelial-Mesenquimal / Factores de Transcripción de la Familia Snail / Neoplasias Renales Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Arch Esp Urol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: España
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vena Cava Inferior / Carcinoma de Células Renales / Progresión de la Enfermedad / Transición Epitelial-Mesenquimal / Factores de Transcripción de la Familia Snail / Neoplasias Renales Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Arch Esp Urol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: España