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Multi-omics analysis revealed that the protein kinase MoKin1 affected the cellular response to endoplasmic reticulum stress in the rice blast fungus, Magnaporthe oryzae.
Zhang, Lianhu; Zhang, Yifan; Liu, Yankun; Miao, Wenjing; Ai, Jingyu; Li, Jingling; Peng, Song; Li, Songyan; Ye, Lifang; Zeng, Rong; Shi, Xugen; Ma, Jian; Lin, Yachun; Kuang, Weigang; Cui, Ruqiang.
Afiliación
  • Zhang L; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Zhang Y; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Liu Y; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Miao W; College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Ai J; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Li J; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Peng S; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Li S; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Ye L; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Zeng R; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Shi X; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Ma J; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.
  • Lin Y; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China. linyachun@jxau.edu.cn.
  • Kuang W; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China. kwing23@126.com.
  • Cui R; College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China. cuiruqiang@jxau.edu.cn.
BMC Genomics ; 25(1): 449, 2024 May 07.
Article en En | MEDLINE | ID: mdl-38714914
ABSTRACT

BACKGROUND:

Previous studies have shown that protein kinase MoKin1 played an important role in the growth, conidiation, germination and pathogenicity in rice blast fungus, Magnaporthe oryzae. ΔMokin1 mutant showed significant phenotypic defects and significantly reduced pathogenicity. However, the internal mechanism of how MoKin1 affected the development of physiology and biochemistry remained unclear in M. oryzae.

RESULT:

This study adopted a multi-omics approach to comprehensively analyze MoKin1 function, and the results showed that MoKin1 affected the cellular response to endoplasmic reticulum stress (ER stress). Proteomic analysis revealed that the downregulated proteins in ΔMokin1 mutant were enriched mainly in the response to ER stress triggered by the unfolded protein. Loss of MoKin1 prevented the ER stress signal from reaching the nucleus. Therefore, the phosphorylation of various proteins regulating the transcription of ER stress-related genes and mRNA translation was significantly downregulated. The insensitivity to ER stress led to metabolic disorders, resulting in a significant shortage of carbohydrates and a low energy supply, which also resulted in severe phenotypic defects in ΔMokin1 mutant. Analysis of MoKin1-interacting proteins indicated that MoKin1 really took participate in the response to ER stress.

CONCLUSION:

Our results showed the important role of protein kinase MoKin1 in regulating cellular response to ER stress, providing a new research direction to reveal the mechanism of MoKin1 affecting pathogenic formation, and to provide theoretical support for the new biological target sites searching and bio-pesticides developing.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Proteínas Fúngicas / Proteómica / Estrés del Retículo Endoplásmico Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Proteínas Fúngicas / Proteómica / Estrés del Retículo Endoplásmico Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido