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Xpert MTB/XDR assay: rapid TB drug resistance detection.
Sethi, Sunil; Sharma, Shelly; Aggarwal, Ashutosh N; Dhatwalia, Sunil Kumar; Rana, Rajesh; Yadav, Rakesh.
Afiliación
  • Sethi S; Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India. sunilsethi10@hotmail.com.
  • Sharma S; Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
  • Aggarwal AN; Pulmonary Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
  • Dhatwalia SK; Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
  • Rana R; State TB Cell, Chandigarh, India.
  • Yadav R; Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Infection ; 2024 May 06.
Article en En | MEDLINE | ID: mdl-38709461
ABSTRACT

PURPOSE:

To assess the Xpert MTB/XDR assay's efficiency in promptly detecting resistance to isoniazid, fluoroquinolones, ethionamide, and second-line injectable drugs among tuberculosis (TB) patients.

METHODS:

From August 2020 to July 2021, TB suspected patient samples were enrolled at a tertiary care center for our study. We conducted mycobacterial culture, phenotypic DST using proportion method in liquid culture at WHO-recommended concentrations, and the line probe assay (LPA). Simultaneously, the Index test, Xpert MTB/XDR, was performed following the manufacturer's instructions.

RESULTS:

Among 360 samples, 107 were excluded due to incomplete information. Resistance to isoniazid, levofloxacin and moxifloxacin was found in 45/251, 21/251 and 20/251 samples, respectively by phenotypic DST. The diagnostic accuracy of Index test, taking phenotypic DST as a reference standard, was 95.8%, 99.04%, and 99.05% for isoniazid, levofloxacin, and moxifloxacin, respectively. The Index test assay demonstrated a specificity of 99.1% for detecting SLID resistance, yielding a diagnostic accuracy of 99.2. Comparing the Index test with LPA revealed a significant enhancement in sensitivity for detecting isoniazid resistance (86.7% vs. 82.2%).

CONCLUSIONS:

The Index test exhibited promising outcomes in identifying resistance to isoniazid and fluoroquinolones, surpassing the performance of the LPA. This could be valuable for promptly initiating treatment in cases of drug-resistant tuberculosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Infection Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Infection Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania