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Corn Silk Polysaccharide Reduces the Risk of Kidney Stone Formation by Reducing Oxidative Stress and Inhibiting COM Crystal Adhesion and Aggregation.
Heng, Bao-Li; Wu, Fan-Yu; Tong, Xin-Yi; Zou, Guo-Jun; Ouyang, Jian-Ming.
Afiliación
  • Heng BL; Yingde Center, Institute of Kidney Surgery, Jinan University, Guangdong 510000, China.
  • Wu FY; Department of Urology, People's Hospital of Yingde City, Yingde 513000, China.
  • Tong XY; Yingde Center, Institute of Kidney Surgery, Jinan University, Guangdong 510000, China.
  • Zou GJ; Department of Urology, People's Hospital of Yingde City, Yingde 513000, China.
  • Ouyang JM; Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.
ACS Omega ; 9(17): 19236-19249, 2024 Apr 30.
Article en En | MEDLINE | ID: mdl-38708219
ABSTRACT
The aim of this study is to explore the inhibition of nanocalcium oxalate monohydrate (nano-COM) crystal adhesion and aggregation on the HK-2 cell surface after the protection of corn silk polysaccharides (CSPs) and the effect of carboxyl group (-COOH) content and polysaccharide concentration.

METHOD:

HK-2 cells were damaged by 100 nm COM crystals to build an injury model. The cells were protected by CSPs with -COOH contents of 3.92% (CSP0) and 16.38% (CCSP3), respectively. The changes in the biochemical indexes of HK-2 cells and the difference in adhesion amount and aggregation degree of nano-COM on the cell surface before and after CSP protection were detected.

RESULTS:

CSP0 and CCSP3 protection can obviously inhibit HK-2 cell damage caused by nano-COM crystals, restore cytoskeleton morphology, reduce intracellular ROS level, inhibit phosphoserine eversion, restore the polarity of the mitochondrial membrane potential, normalize the cell cycle process, and reduce the expression of adhesion molecules, OPN, Annexin A1, HSP90, HAS3, and CD44 on the cell surface. Finally, the adhesion and aggregation of nano-COM crystals on the cell surface were effectively inhibited. The carboxymethylated CSP3 exhibited a higher protective effect on cells than the original CSP0, and cell viability was further improved with the increase in polysaccharide concentration.

CONCLUSIONS:

CSPs can protect HK-2 cells from calcium oxalate crystal damage and effectively reduce the adhesion and aggregation of nano-COM crystals on the cell surface, which is conducive to inhibiting the formation of calcium oxalate kidney stones.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Omega Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Omega Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos