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The BioCascade Impactor: A novel device for direct collection of size-fractionated bioaerosols into liquid medium.
Chen, Yuqiao; Chen, Jiayi; Shankar, Sripriya Nannu; Amanatidis, Stavros; Eiguren-Fernandez, Arantzazu; Kreisberg, Nathan; Spielman, Steven; Lednicky, John A; Wu, Chang-Yu.
Afiliación
  • Chen Y; Department of Environmental Engineering Sciences, College of Engineering, University of Florida, Gainesville, Floida, USA.
  • Chen J; Department of Environmental Engineering Sciences, College of Engineering, University of Florida, Gainesville, Floida, USA.
  • Shankar SN; Department of Environmental Engineering Sciences, College of Engineering, University of Florida, Gainesville, Floida, USA.
  • Amanatidis S; Department of Environmental and Public Health Sciences, College of Medicine, University of Cincinnati, Ohio, USA.
  • Eiguren-Fernandez A; Aerosol Dynamics Inc., Berkeley, California, USA.
  • Kreisberg N; Aerosol Dynamics Inc., Berkeley, California, USA.
  • Spielman S; Aerosol Dynamics Inc., Berkeley, California, USA.
  • Lednicky JA; Aerosol Dynamics Inc., Berkeley, California, USA.
  • Wu CY; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA.
Aerosol Sci Technol ; 58(3): 264-275, 2024.
Article en En | MEDLINE | ID: mdl-38706712
ABSTRACT
The ability to collect size-fractionated airborne particles that contain viable bacteria and fungi directly into liquid medium while also maintaining their viability is critical for assessing exposure risks. In this study, we present the BioCascade impactor, a novel device designed to collect airborne particles into liquid based on their aerodynamic diameter in three sequential stages (>9.74 µm, 3.94-9.74 µm, and 1.38-3.94 µm when operated at 8.5 L/min). Aerosol samples containing microorganisms - either Saccharomyces kudriavzevii or Micrococcus luteus, were used to evaluate the performance of the BioCascade (BC) paired with either the VIable Virus Aerosol Sampler (VIVAS) or a gelatin filter (GF) as stage 4 to collect particles <1.38 µm. Stages 2 and 3 collected the largest fractions of viable S. kudriavzevii when paired with VIVAS (0.468) and GF (0.519), respectively. Stage 3 collected the largest fraction of viable M. luteus particles in both BC+VIVAS (0.791) and BC+GF (0.950) configurations. The distribution function of viable microorganisms was consistent with the size distributions measured by the Aerodynamic Particle Sizer. Testing with both bioaerosol species confirmed no internal loss and no re-aerosolization occurred within the BC. Irrespective of the bioaerosol tested, stages 1, 3 and 4 maintained ≥80% of viability, while stage 2 maintained only 37% and 73% of viable S. kudriavzevii and M. luteus, respectively. The low viability that occurred in stage 2 warrants further investigation. Our work shows that the BC can efficiently size-classify and collect bioaerosols without re-aerosolization and effectively maintain the viability of collected microorganisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Aerosol Sci Technol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Aerosol Sci Technol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos