Your browser doesn't support javascript.
loading
Comparison of Three Widely Employed Extraction Methods for Metabolomic Analysis of Trypanosoma brucei.
Fall, Fanta; Desmet, Lieven; Mamede, Lúcia; Schioppa, Laura; de Tullio, Pascal; Frédérich, Michel; Govaerts, Bernadette; Quetin-Leclercq, Joëlle.
Afiliación
  • Fall F; Pharmacognosy Research Group, Louvain Drug Research Institute (LDRI), Université Catholique de Louvain (UCLouvain), Brussels, Belgium.
  • Desmet L; Institute of Statistics, Biostatistics and Actuarial Sciences (ISBA/LIDAM), UCLouvain, Louvain-la-Neuve, Belgium.
  • Mamede L; Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines (CIRM), University of Liège, Belgium.
  • Schioppa L; Pharmacognosy Research Group, Louvain Drug Research Institute (LDRI), Université Catholique de Louvain (UCLouvain), Brussels, Belgium.
  • de Tullio P; Metabolomics group, Center for Interdisciplinary Research on Medicines (CIRM), University of Liège, Belgium.
  • Frédérich M; Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines (CIRM), University of Liège, Belgium.
  • Govaerts B; Institute of Statistics, Biostatistics and Actuarial Sciences (ISBA/LIDAM), UCLouvain, Louvain-la-Neuve, Belgium.
  • Quetin-Leclercq J; Pharmacognosy Research Group, Louvain Drug Research Institute (LDRI), Université Catholique de Louvain (UCLouvain), Brussels, Belgium.
Curr Protoc ; 4(5): e1043, 2024 May.
Article en En | MEDLINE | ID: mdl-38706422
ABSTRACT
Trypanosoma brucei (Tb) is the causative agent of human African trypanosomiasis (HAT), also known as sleeping sickness, which can be fatal if left untreated. An understanding of the parasite's cellular metabolism is vital for the discovery of new antitrypanosomal drugs and for disease eradication. Metabolomics can be used to analyze numerous metabolic pathways described as essential to Tb. brucei but has some limitations linked to the metabolites' physicochemical properties and the extraction process. To develop an optimized method for extracting and analyzing Tb. brucei metabolites, we tested the three most commonly used extraction methods, analyzed the extracts by hydrophilic interaction liquid chromatography high-resolution mass spectrometry (HILIC LC-HRMS), and further evaluated the results using quantitative criteria including the number, intensity, reproducibility, and variability of features, as well as qualitative criteria such as the specific coverage of relevant metabolites. Here, we present the resulting protocols for untargeted metabolomic analysis of Tb. brucei using (HILIC LC-HRMS). © 2024 Wiley Periodicals LLC. Basic Protocol 1 Culture of Trypanosoma brucei brucei parasites Basic Protocol 2 Preparation of samples for metabolomic analysis of Trypanosoma brucei brucei Basic Protocol 3 LC-HRMS-based metabolomic data analysis of Trypanosoma brucei brucei.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma brucei brucei / Metabolómica Idioma: En Revista: Curr Protoc Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma brucei brucei / Metabolómica Idioma: En Revista: Curr Protoc Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos