Correlative light and electron microscopy at defined cell cycle stages in a controlled environment.
Methods Cell Biol
; 187: 73-97, 2024.
Article
en En
| MEDLINE
| ID: mdl-38705631
ABSTRACT
Cells are dynamic machines that continuously change their architecture to adapt and respond to extracellular and intracellular stimuli. Deciphering dynamic processes with nanometer-scale resolution inside cells is critical for mechanistic understanding. Here, we present a protocol that enables the in situ study of dynamic changes in intracellular structures under close-to-native conditions at high spatiotemporal resolution. Importantly, the cells are grown, transported, and imaged in a chamber in which environmental conditions such as temperature and gas (e.g., carbon dioxide or oxygen) concentration can be controlled. We demonstrate this protocol to quantify ultrastructural changes that occur during the cell cycle of cultured mammalian cells. The environment control system opens up the possibility of applying this method to primary cells, tissues, and organoids by adjusting environmental conditions.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ciclo Celular
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Cell Biol
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Estados Unidos